Phytochemical profiling of petroleum ether and chloroform extracts of Curcuma caesia rhizome by GC-MS and comparing their bioactivities
GCMS profiling and bioactivities of Curcuma caesia rhizome
DOI:
https://doi.org/10.25081/josac.2021.v30.i2.7263Keywords:
Phytoconstituents, GC-MS, Bioactivities, antifungal, antibacterial, DPPH scavengingAbstract
The objective of this study was to characterise the putative phytochemical constituents from petroleum ether (CC-P) and chloroform (CC-C) extracts of Curcuma. caesia rhizome by GC-MS analysis and to compare their bioactivities. GC-MS analysis facilitated the documentation of a total of 16 and 20 volatile constituents in CC-P and CC-C extracts respectively. Of these the major constituents present in CC-P were cycloisolongifolence, 8, 9-dehydro-9 formyl- (19.64%), 4-oxo- β-isodamascol (16.05%), 6,10-dimethyl-3- (1-methylethyl) -6-cyclodecene-1,4-dione (13.80%), 2H-cyclohepta[b]furan-2-one,6-[1-(acetyloxy) -3-oxobutyl]- 3,3a,4,7,8,8a- hexahydro-7-methyl- 3-methylene- (9.08%), tricycle [5.1.0.0(2,4)] oct-5-ene-5-propanoic acid,3,3,8.8-tetramethyl- (6.39%), 2(3H)-benzofuranone,6-ethenylhexahydro-3,6-dimethyl-7-(1- methyl ethenyl)-, [3S-(3α,3aα,6α,7β,7aβ) (4.18%) and 7a-isopropenyl-4,5-dimethy loctahydroindene-4-carboxylic acid (4.01%). Similarly, the major constituents present in CC-C were cycloisolongifolence,8,9- dehydro-9-formyl-(15.69%), 6,10-dimethyl-3-(1-methylethyl)-6-cyclodecene-1, 4-dione (13.38%), 2H-cyclohepta [b]furan-2-one,6-[1- (acetyloxy)-3-oxobutyl] -3,3a,4,7,8,8a- hexa hydro -7-methyl - 3-methylene- (4.55%), tricycle [5.1.0.0(2,4)] oct-5-ene-5- propanoic acid,3,3,8.8-tetramethyl- (7.50%), 2(3H)-benzofuranone, 6-ethenylhexahydro-3,6-dimethyl-7-(1-methylethenyl)-,[3S- (3α,3aα,6α,7β,7aβ) (6.15%), 7a-isopropenyl-4,5-dimethyl octahydroindene-4-carboxylic acid (6.43%) and 2-(4a,8- Dimethyl-6- oxo-1,2,3,4-4a,5,6,8a-octahydro-naphthalen-2-yl) propionaldehyde (6.57%). The extracts, CC-P and CC-C exhibited almost similar antibacterial and antifungal potentials but differed with respect to antioxidant activity. The IC50 values of CC-P and CC-C extracts for DPPH scavenging were 0.68±0.02 mg ml-1 and 0.13±0.01 mg ml-1 respectively. Together, above results provide an important basis for the isolation of bioactive compounds from C. caesia rhizome extract and their use as traditional medicine.
Downloads
References
Aiyelaagbe OO & O samudiamen PM 2009 Phytochemical screening for active compounds in Mangifera indica from Ibadan, Oyo State. Plant Sci. Res.2:11–13.
Arulmozhi DK, Sridhar N, Veeranjaneyulu A & Arora SK 2006 Preliminary mechanistic studies on the smooth muscle relaxant effect of hydroalcoholic extract of Curcuma caesia. J. Herbal Pharmacotherapy 6(3/4):117–124.
Astutik S, Jürgen P & Jude NK 2019 Asian medicinal plants’ production and utilization potentials: are view. Sustainability 11:5483.
Autom R S, Warjeet S L, Rakhi K H, Shaukat AMS, Raghumani S N, Kunwar A 2021 Evaluation of phytochemical, antioxidant and antimicrobial activities of Curcumaleucorrhiza (Zingiberaceae) Roxb. rhizome. IJPSR 12(9): 4764-4773.
Azwanida N N 2015 A Review on the extraction methods use in medicinal plants, principle, strength and limitation. Med. Aromat. Plants 4: 196.
Banerjee A & Nigam SS 1976 Antifungal activity of the essential oil of Curcuma caesia Roxb. Indian J. Med. Res.64: 1318-1321.
Bauer A W, Kirby W M M, Sherris J C & Turck M 1966 Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol.45: 493–496.
Bhupendra S, Rameshroo K & Shashikant C 2016 Medicinal value of Curcuma cassia Roxb: an overview.UK J. Pharm.Biosci.4 (6): 69-74.
Borah A, Paw M, Gogoi R, Loying R, Sarma N, Munda S, Pandey S K & Lal M 2019 Chemical composition, antioxidant, anti-inflammatory, anti-microbial and invitro cytotoxic efficacy of essential oil of Curcuma caesia Roxb. leaves: an endangered medicinal plant of north east India. Ind. Crops. Prod.129: 448-454.
Chadwick M, Trewin H, Gawthrop F & Wagstaff C 2013 Sesquiterpenoids lactones: benefits to plants and people. Int. J. Mol. Sci. 14(6): 12780–12805.
HeisanamP, Mazumder PB & Devi LP 2015 Antioxidant and antimutagenic activity of Curcuma caesia Roxb. rhizome extracts. Toxicol. Rep. 2:423–428.
Donipati P & Sreeramulu S H 2015 Preliminary phytochemical screening of Curcuma caesia.Int. J.Curr.Microbiol. App. Sci.4 (11): 30–34.
Gupta VK, Kaushik A, Chauhan DS, Ahirwar RK, Sharma S, Bisht D 2018 Anti- mycobacterial activity of some medicinal plants used traditionally by tribes from Madhya Pradesh, India for treating tuberculosis related symptoms. J. Ethnopharmacol.227:113–120.
I-Nan C, Chen-Chin C, Chang-Chai N, Chung-Yi W, Yuan-Tay S, Tsu-Liang C 2008 Antioxidant and antimicrobial activity of Zingiberaceae plants in Taiwan. Plant Foods Hum.Nutr.63:15–20.
Jain A, Parihar DK 2018 In vitro antioxidant and antidiabetic activity of regional chemotypes of three Curcuma species from Chhattisgarh. J. Herb. Sci.7:13–22.
Jose S & Thomas TD 2014 Comparative phytochemical and anti-bacterial studies of two indigenous medicinal plants Curcuma caesia Roxb. and Curcuma aeruginosa Roxb. Int. J. Green Pharm.8:65–71.
Kamazeri T S, Samah O A, Taher M, Susanti D & Qaralleh H 2012 Antimicrobial activity andessential oils of Curcuma aeruginosa, Curcuma mangga, and Zingiber cassumunar from Malaysia. Asian. Pac. J. Trop. Med. 5: 202-209.
Karmakar I, Dolai N,Suresh Kumar RB,Kar B, Roy SN&Haldar PK 2013 Antitumor activity and antioxidant property of Curcuma caesia against Ehrlich’s ascites carcinoma bearing mice. Pharm Biol 2013; 51(6): 753–759.
Lai EY, Chyau CC, Mau JL, Chen CC, Lai YJ, Shih CF & Lin L L2004 Antimicrobial activity and cytotoxicity of the essential oil of Curcuma zedoaria. Am. J. Chin. Med. 32: 281–90.
Lakshmi S, Padmaja G&Remani P 2011 Antitumour effects of isocurcumenol isolated from Curcuma zedoaria rhizomes on human and murine cancer cells. Int. J. Med. Chem. 2011: 253962.
Mukunthan KS, Kumar ANV,Balaji S& Trupti NP. 2014 Analysis of essential oilconstituents in rhizome of Curcuma caesia Roxb. from south India. J. Essent. Oil-Bear. Plants 17:647–651.
Nethathe BB & N dip RN 2011 Bioactivity of Hydnoraafricana on selected bacterial pathogens: preliminary phytochemical screening. Afr. J. Microbiol. Res.518: 2820–2826.
Pancharoen O, Prawat U, Tuntiwachwuttikul P 2000 Phytochemistry of the zingiberaceae. Stud. Nat. Prod. Chem. 23: 797–865.
Paw M, Gogoi R,Sarma N, Pandey SK, Borah A, Begun T, & Lal M 2020 Study of antioxidant, anti-inflammatory, genotoxicity, antimicrobial activities and analysis of different constituents found in rhizome essential oil of Curcuma caesia Roxb, collected from northeast India. Curr. Pharm. Biotechnol.21 (5):403–413.
Satyavam D A & Warjeet SL 2012 Investigation of the structure-non linearity relationship of zederone from rhizome of Curcuma caecia Roxb. Indian J. Chem.51:1738–1742.
Rita de Cássia da Silveira e Sá, Andrade L N & de Sousa D P 2015 Sesquiterpenes from essential oils and anti-inflammatory activity. Nat. Prod.Commun. 10 (10): 1767–1774.
Sastri B N 1962 The wealth of India - Raw materials. CSIR New Delhi.
Singh H, Krishna G, Baske PK 2010 Plants used in the treatment of joint diseases (rheumatism, arthritis, gout and lumbago) in Mayurbhanj district of Odisha, India. Report and Opinion 2(9): 22–26.
Sonjit D, Prodyut M, Zaman M K 2013 Curcuma caesiaRoxb. and it’s medicinal uses: a review. IJRPC3 (2): 370–375.
Voravuthikunchai S P 2007 Family Zingiberaceae compounds as functional antimicrobials, antioxidants, and antiradicals. Food Global Science Books 1 (2): 227–240.
Published
How to Cite
Issue
Section
Copyright (c) 2022 Rameshor S. Atom, Shaukat Ali M. Shaikh, Warjeet S. Laitonjam, Raghumani S. Ninghthoujam, Amit Kunwar
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
.