Induction of somatic embryogenesis and genetic fidelity of endangered medicinal herb Curculigo orchioides Gaertn

Authors

  • Swati Patel C.G.Bhakta Institute of biotechnology, Gopal Vidhyanagar, Bardoli, Surat-394350
  • Yogesh T Jasrai Department of Botany, University School of Science, Gujarat University, Ahmedabad-380009, Gujarat, India
  • Roshni Adiyecha Department of Botany, University School of Science, Gujarat University, Ahmedabad-380009, Gujarat, India

Keywords:

Somatic, embryogenesis, fidelity

Abstract

An efficient regeneration system, through somatic embryogenesis was developed for Curculigo orchioides Gaertn - an endangered medicinal herb. Somatic embryos weredeveloped on MS medium containing 8 - 15μM BA from leaf explants. The highest, 69 % leafexplants responded in terms of embryogenic calli with average 8 embryos on MS mediumcontaining 8μM BA. Regenerated plantlets were transferred to autoclaved mixture of soil:sand: compost (1:1:1; v/v/v) for hardening. Genetic fidelity of somatic embryogenesis derivedregenerant was assessed using random amplified polymorphic DNA (RAPD).

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Published

15-08-2011

How to Cite

Patel, S., Jasrai, Y. T., & Adiyecha, R. (2011). Induction of somatic embryogenesis and genetic fidelity of endangered medicinal herb Curculigo orchioides Gaertn. Research in Plant Biology, 1(3). Retrieved from https://www.updatepublishing.com/journal/index.php/ripb/article/view/2583

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