Somatic embryogenesis and plant regeneration from cotyledon tissue of Arachis hypogaea L.

Abstract

Tissue culture techniques are useful for ex situ conservation of rare, endemic orthreatened plant species. This report describes a protocol for somatic embryogenesis ofpeanut (Arachis hypogaea L.) through cotyledon explants. Only 10% of the explants werecontaminated by using this material to start the in vitro culture. The highest frequency(35.4%) of embryogenic calli induction was observed on MS medium supplemented with0.50 mg/l KIN in combination with 0.10 mg/l 2,4,-D. the percentage response for embryogenicproliferation increased in the medium supplemented with GA3(0.20 mg/l) and 1.0 mg/l BA.The regenerated embryos were successfully transferred to the embryo development mediumand transferred to the trays after the development of secondary and tertiary rootdevelopment.