Effects of 2, 4-Dichlorophenoxyacetic Acid on the in vitro growth of explants (Clerodendron spp.)

Abstract

Callus cultures and suspension cultures began with pieces of tissues and cells fromthe leaf but culture conditions were manipulated to create an unorganized state. Theinitiation of callus was induced from dicotyledonous tissue explants (Clerodendron spp.) firstby preparing MS basal medium and then by adding varying concentrations of 2, 4-Dichlorophenoxyacetic Acid or 2, 4-D. Growth was observed after each week for a period of3 weeks and observations were documented. After all the observations were noted down;subsequent analysis and interpretation of the results followed. This led to a valid scientificconclusion for the whole study process. Though there was disturbance due to heavy bacterialcontamination in majority of the petriplates, but the effect of plant-status and environmentalfactors on callus initiation cannot be fully ignored. Growth response studied was morevigorous in low concentrations of 2, 4-D as compared to higher levels.