A set of variable plastid SSR markers for the genus Cryptanthus (Bromeliaceae)

Authors

  • Florian Krapp Plant Molecular Systematics, Department of Sciences, University of Kassel, D-34132 Kassel, Germany
  • Geyner Alves dos Santos Cruz Genetics Department, CCB, Universidade Federal de Pernambuco (UFPE), Av. Prof. Moraes Rego, 1235, 50.670-420, Recife, PE, Brazil
  • Tina Wöhrmann Plant Molecular Systematics, Department of Sciences, University of Kassel, D-34132 Kassel, Germany
  • Ana Maria Benko-Iseppon Genetics Department, CCB, Universidade Federal de Pernambuco (UFPE), Av. Prof. Moraes Rego, 1235, 50.670-420, Recife, PE, Brazil
  • Kurt Weising Plant Molecular Systematics, Department of Sciences, University of Kassel, D-34132 Kassel, Germany

Keywords:

Bromeliaceae, Bromelioideae, Cryptanthus, cpSSR, microsatellites, population genetics

Abstract

The genus Cryptanthus (Bromeliaceae) is endemic to Brazil. Many of its currentlyrecognized 66 species are narrow endemics that are threatened by habitat destruction.Molecular markers are needed to evaluate the extent and distribution of genetic diversityin rare Cryptanthus species, which would be a prerequisite for taking appropriateconservation measures. Here we describe the development of plastid microsatellitemarkers (cpSSRs) for Cryptanthus. PCR primers specific for 34 cpSSR loci in Dyckiamarnier-lapostollei were initially tested for their functionality in Cryptanthus schwackeanus. PCR was successful for 29 loci, and 13 loci were shown to harbour extended stretches of mononucleotide repeats. Seven loci were further characterized bygenotyping Cryptanthus samples at the level of populations and species, and six lociproved to be polymorphic among 30 individuals of each of the two endangered species C. schwackeanus and C. warren-loosei, respectively. All primers cross-amplified in other genera from three subfamilies of Bromeliaceae.

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Published

08-04-2013

How to Cite

Krapp, F., dos Santos Cruz, G. A., Wöhrmann, T., Benko-Iseppon, A. M., & Weising, K. (2013). A set of variable plastid SSR markers for the genus Cryptanthus (Bromeliaceae). Research in Plant Biology, 3(2). Retrieved from https://www.updatepublishing.com/journal/index.php/ripb/article/view/2521

Issue

Vol. 3 No. 2 (2013)

Section

Short Communications