Impacts of F2 derived winter bread wheat progenies on callus production and regeneration frequencies through anther culture

Abstract

Anther culture is one of the most feasible techniques to obtain doubled haploid(DH) plants. In this study, anther culture responses of F2 derived bread wheat hybrids ofYektay406 × Altay 2000, Demir2000 × Soyer02, Atlı2002 × Müfitbey, Müfitbey × Atlı2002,Müfitbey × Eser, Müfitbey × Bezostaya were assessed to clarify the genotype-basedreactions under tissue culture conditions. Firstly, twenty five spikes which were grown indry land, collected from F2 hybrid plants. A total of 10.963 inoculated F2 anthers formed to188 callus in the primary hormone-free MN6 media. Some of the calli produced hybridgroups did not generate any haploid plantlet in regeneration media. Regenerationfrequencies of Demir2000 × Soyer02 and Müfitbey × Atlı2002 hybrids were 30.76% and63.63% respectively. F2 anthers of Müfitbey × Atlı2002 produced 121 callus, 10 albino and77 haploid plantlets. In contrary, Atlı2002 × Müfitbey produced only 26 callus, 3 albinoand zero haploid plantlets. Other corresponding hybrids of Müfitbey × Eser and Müfitbey× Bezostaya generated 9 and 13 callus, while the least number of callus was detected inYektay406 × Altay 2000. A correlation between callus number and haploid plantletnumber per 100 anther revealed a positive relationship for each F2 progeny (r = 0.988, P <0.01). Our results significantly indicated that Müfitbey cultivar might be used an effectiveanther source for DH wheat production.