Histomorphological effects of ß,Ɛ-Carotene-3,3’-diol on the liver following indomethacin-induced hepatotoxicity in adult male Wistar rats
DOI:
https://doi.org/10.25081/jmhe.2024.v10.9014Keywords:
ß,Ɛ-Carotene-3,3’-diol, Liver, Indomethacin, Histopathology, Medicinal PlantsAbstract
Medicinal plants are globally used as food and in the management of different ailments based on their peculiar phytochemical contents. This study aimed to evaluate the histomorphological effects of ß,Ɛ-Carotene-3,3’-diol on the liver following indomethacin-induced hepatotoxicity in adult male Wistar rats. This is to determine the ameliorative effect of graded doses of ß,Ɛ-Carotene-3,3’-diol. A total of twenty-five adult Wistar rats were randomly divided into five groups (n=5). Liver injury was induced by oral administration of 20 mg/kg b.w of indomethacin after 24 hours fast to animals in groups B-E. Twenty four hours after the induction, animals in groups C-E were given different doses of ß,Ɛ-Carotene-3,3’-diol. At the end of the experimentation, the animals were sacrificed and the livers were collected and fixed for histopathological and histomorphological analyses. The results showed that indomethacin increased the activities of AST, ALT and ALP and also induced histopathological changes. Treatment with ß,Ɛ-Carotene-3,3’-diol was able to attenuate liver injury caused by indomethacin in a dose dependent manner as evidenced in the histoarchitectural changes. This was also corroborated by the histomorphometric analyses. In conclusion, the results suggested the positive influence of ß,Ɛ-Carotene-3,3’-diol in ameliorating liver injury caused by indomethacin and by extension, may be adopted in the management of liver related injury.
Downloads
References
Amen, Y., Sherif, A. E., Shawky, N. M., Abdelrahman, R. S., Wink, M., & Sobeh, M. (2020). Grape-Leaf Extract Attenuates Alcohol-Induced Liver Injury via Interference with NF-κB Signaling Pathway. Biomolecules, 10(4), 558. https://doi.org/10.3390/biom10040558
Antar, S. A., Ashour, N. A., Marawan, M. E., & Al-Karmalawy, A. A. (2023). Fibrosis: Types, Effects, Markers, Mechanisms for Disease Progression, and Its Relation with Oxidative Stress, Immunity, and Inflammation. International Journal of Molecular Sciences, 24(4), 4004. https://doi.org/10.3390/ijms24044004
Basivireddy, J., Jacob, M., Pulimood, A. B., & Balasubramanian, K. A. (2004). Indomethacin-induced renal damage: role of oxygen free radicals. Biochemical Pharmacology, 67(3), 587-599. https://doi.org/10.1016/j.bcp.2003.09.023
Bhattacharyya, S., Datta, S., Mallick, B., Dhar, P., & Ghosh, S. (2010). Lutein content and in vitro antioxidant activity of different cultivars of Indian marigold flower (Tagetes patula L.) extracts. Journal of Agricultural and Food Chemistry, 58(14), 8259-8264. https://doi.org/10.1021/jf101262e
Chen, P., Chen, C., Hu, M., Cui, R., Liu, F., Yu, H., & Ren, Y. (2020). S-allyl-L-cysteine protects hepatocytes from indomethacin-induced apoptosis by attenuating endoplasmic reticulum stress. FEBS Open Bio, 10(9), 1900-1911. https://doi.org/10.1002/2211-5463.12945
Cherkas, Y., McMillian, M. K., Amaratunga, D., Raghavan, N., & Sasaki, J. C. (2016). ABC gene-ranking for prediction of drug-induced cholestasis in rats. Toxicology Reports, 3, 252-261. https://doi.org/10.1016/j.toxrep.2016.01.009
Devarbhavi, H., Asrani, S. K., Arab, J. P., Nartey, Y. A., Pose, E., & Kamath, P. S. (2023). Global burden of liver disease: 2023 update. Journal of Hepatology, 79(2), 516-537. https://doi.org/10.1016/j.jhep.2023.03.017
Drożdżal, S., Lechowicz, K., Szostak, B., Rosik, J., Kotfis, K., Machoy-Mokrzyńska, A., Białecka, M., Ciechanowski, K., & Gawrońska-Szklarz, B. (2021). Kidney damage from nonsteroidal anti-inflammatory drugs-Myth or truth? Review of selected literature. Pharmacology Research & Perspectives, 9(4), e00817. https://doi.org/10.1002/prp2.817
Ejaz, P., Bhojani, K., & Joshi, V. R. (2004). NSAIDs and kidney. The Journal of the Association of Physicians of India, 52, 632-640.
Erdman, J. W., Jr., Smith, J. W., Kuchan, M. J., Mohn, E. S., Johnson, E. J., Rubakhin, S. S., Wang, L., Sweedler, J. V., & Neuringer, M. (2015). Lutein and Brain Function. Foods, 4(4), 547-564. https://doi.org/10.3390/foods4040547
Farzaneh, V., & Carvalho, I. S. (2015). A review of the health benefit potentials of herbal plant infusions and their mechanism of actions. Industrial Crops and Products, 65, 247-258. https://doi.org/10.1016/j.indcrop.2014.10.057
Goldberg, E., Chopra, S., & Rubin, J. N. (2018). Acute liver failure in adults: etiology, clinical manifestations, and diagnosis. Waltham, MA, USA: UpToDate.
Gunaydin, C., & Bilge, S. S. (2018). Effects of Nonsteroidal Anti-Inflammatory Drugs at the Molecular Level. The Eurasian Journal of Medicine, 50(2), 116-121. https://doi.org/10.5152/eurasianjmed.2018.0010
Herminghaus, A., Buitenhuis, A. J., Schulz, J., Truse, R., Vollmer, C., Relja, B., Bauer, I., & Picker, O. (2020). Indomethacin Increases the Efficacy of Oxygen Utilization of Colonic Mitochondria and Uncouples Hepatic Mitochondria in Tissue Homogenates From Healthy Rats. Frontiers in Medicine, 7, 463. https://doi.org/10.3389/fmed.2020.00463
Johnson, E. J. (2014). Role of lutein and zeaxanthin in visual and cognitive function throughout the lifespan. Nutrition Reviews, 72(9), 605-612. https://doi.org/10.1111/nure.12133
Kawata, A., Murakami, Y., Suzuki, S., & Fujisawa, S. (2018). Anti-inflammatory Activity of β-Carotene, Lycopene and Tri-n-butylborane, a Scavenger of Reactive Oxygen Species. In Vivo, 32(2), 255-264. https://doi.org/10.21873/invivo.11232
Khan, S., Yusufi, F. N. K., & Yusufi, A. N. K. (2019). Comparative effect of indomethacin (IndoM) on the enzymes of carbohydrate metabolism, brush border membrane and oxidative stress in the kidney, small intestine and liver of rats. Toxicology Reports, 6, 389-394. https://doi.org/10.1016/j.toxrep.2019.04.010
Kumar, G., Banu, G. S., Kannan, V., & Pandian, M. R. (2005). Antihepatotoxic effect of beta-carotene on paracetamol induced hepatic damage in rats. Indian Journal of Experimental Biology, 43(4), 351-355.
Mahmoud, S. H., Elshaer, A. M., El-Waseef, D. A., Erfan, D. M., Nabih, E. S., El-Kharashi, O. A., & AboZeid, M. S. (2021). Effect of indomethacin (Non steroidal anti-infilammatory drug) on intestinal permeability and liver functions. QJM: An International Journal of Medicine, 114(S1), hcab114-005. https://doi.org/10.1093/qjmed/hcab114.005
Mansourian, M., Mirzaei, A., Azarmehr, N., Vakilpour, H., Kokhdan, E. P., & Doustimotlagh, A. H. (2019). Hepatoprotective and antioxidant activity of hydroalcoholic extract of Stachys pilifera. Benth on acetaminophen-induced liver toxicity in male rats. Heliyon, 5(12), e03029. https://doi.org/10.1016/j.heliyon.2019.e03029
Mehta, N., Ozick, L. A., Gbadehan, E. (2022). Drug-Induced Hepatotoxicity. Emergency Medicine. Medscape. Retrieved from https://emedicine.medscape.com/article/169814-overview?form=fpf
Ofusori, D. A., Ofusori, A. E., Awoniran, P. O., Oke, G. O., Abayomi, T. A., & Edward, T. A. (2020). Microstructural effects of Celosia trigyna leave extracts on the liver and ileum in ethanol-induced toxicity in adult Wistar rats. Research Journal of Medicinal Plants, 14(2), 64-71.
Oke, G. O., Abiodun, A. A., Imafidon, C. E., & Monsi, B. F. (2019). Zingiber officinale (Roscoe) mitigates CCl4-induced liver histopathology and biochemical derangements through antioxidant, membrane-stabilizing and tissue-regenerating potentials. Toxicology Reports, 6, 416-425. https://doi.org/10.1016/j.toxrep.2019.05.001
Owojuyigbe, O. S., Larbie, C., Firempong, C. K., Komlaga, G., Emikpe, B. O., & Otuechere, C. A. (2020). Extracts of Hura crepitans L. stem bark attenuate liver injury and inflammation induced by CCl4 in rats. Comparative Clinical Pathology, 29, 1199-1208. https://doi.org/10.1007/s00580-020-03172-2
Perron, N., Tremblay, E., Ferretti, E., Babakissa, C., Seidman, E. G., Levy, E., Ménard, D., & Beaulieu, J.-F. (2013). Deleterious effects of indomethacin in the mid-gestation human intestine. Genomics, 101(3), 171-177. https://doi.org/10.1016/j.ygeno.2012.12.003
Randhawa, M. A., Khan, A. A., Javed, M. S., & Sajid, M. W. (2015). Green leafy vegetables: a health promoting source. In R. R. Watson (Eds.), Handbook of fertility (pp. 205-220) Cambridge, US: Academic Press. https://doi.org/10.1016/B978-0-12-800872-0.00018-4
Rudnick, D. A., Shikapwashya, O., Blomenkamp, K., & Teckman, J. H. (2006). Indomethacin increases liver damage in a murine model of liver injury from alpha-1-antitrypsin deficiency. Hepatology, 44(4), 976-982. https://doi.org/10.1002/hep.21326
Sabiu, S., Garuba, T., Sunmonu, T., Ajani, E., Sulyman, A., Nurain, I., & Balogun, A. (2015). Indomethacin-induced gastric ulceration in rats: Protective roles of Spondias mombin and Ficus exasperata. Toxicology Reports, 2, 261-267. https://doi.org/10.1016/j.toxrep.2015.01.002
Sohail, R., Mathew, M., Patel, K. K., Reddy, S. A., Haider, Z., Naria, M., Habib, A., Abdin, Z. U., Chaudhry, W. R., & Akbar, A. (2023). Effects of Non-steroidal Anti-inflammatory Drugs (NSAIDs) and Gastroprotective NSAIDs on the Gastrointestinal Tract: A Narrative Review. Cureus, 15(4), e37080. https://doi.org/10.7759/cureus.37080
Taiwo, V. O., & Conteh, O. L. (2008). The rodenticidal effect of indomethacin: pathogenesis and pathology. Veterinarski Arhiv, 78(2), 167-178.
Vardi, N., Parlakpinar, H., Cetin, A., Erdogan, A., & Ozturk, I. C. (2010). Protective effect of beta-carotene on methotrexate-induced oxidative liver damage. Toxicologic Pathology, 38(4), 592-597. https://doi.org/10.1177/0192623310367806
Vardi, N., Parlakpinar, H., Ozturk, F., Ates, B., Gul, M., Cetin, A., Erdogan, A., & Otlu, A. (2008). Potent protective effect of apricot and beta-carotene on methotrexate-induced intestinal oxidative damage in rats. Food and Chemical Toxicology, 46(9), 3015-3022. https://doi.org/10.1016/j.fct.2008.05.039
Woolbright, B. L., & Jaeschke, H. (2017). Role of the inflammasome in acetaminophen-induced liver injury and acute liver failure. Journal of Hepatology, 66(4), 836-848. https://doi.org/10.1016/j.jhep.2016.11.017
Wu, S., Chen, R., Chen, J., Yang, N., Li, K., Zhang, Z., & Zhang, R. (2023). Study of the Anti-Inflammatory Mechanism of β-Carotene Based on Network Pharmacology. Molecules, 28(22), 7540. https://doi.org/10.3390/molecules28227540
Zerfu, T. A., Pinto, E., & Baye, K. (2018). Consumption of dairy, fruits and dark green leafy vegetables is associated with lower risk of adverse pregnancy outcomes (APO): a prospective cohort study in rural Ethiopia. Nutrition & Diabetes, 8, 52. https://doi.org/10.1038/s41387-018-0060-y
Published
How to Cite
Issue
Section
Copyright (c) 2024 Adebimpe Esther Ofusori, Yanuardi Raharjo, David Adesanya Ofusori, Victory Opeyemi Adekunle
This work is licensed under a Creative Commons Attribution 4.0 International License.
. 