Qualitative and quantitative metabolomics of Pamburus missionis Swingle – a medicinal tree taxon
DOI:
https://doi.org/10.25081/jmhe.2024.v10.8830Keywords:
Metabolomics, Pamburus missionis, Medicinal plant, PhytochemicalsAbstract
Since ancient times, Plant based medicine is a well-known oldest form of healthcare to mankind. Even the commercial medications used for the treatment of various ailments today, contains a large proportion of plant derived chemical compounds. Hence chemical profiling of medicinal plants gained a key role to use them in pharmaceuticals as well as commercial industries. Pamburus missionis is one of the medicinal plants used in the Indian and Srilankan traditional medicine system to reduce kapha dosha. The ayurvedic physicians used its leaves to treat swelling, piles, fractures, fistula, puerperal diseases and as serpent venom. Therefore, the present study was undertaken to reveal the phytocompounds from various parts of this plant. Metabolomics revealed the presence of primary and secondary metabolites like carbohydrates, lipids, proteins, alkaloids, phenols, flavonoids, steroids etc. From the aqueous extract, Alkaloid content is almost same in leaf, stem and bark extracts with around 1.2 to 1.3 g (w/v%) and completely absent in the fruit. Phenolic content and steroids are notably high in leaf extract with 112.77±0.34 mg/g of extract and 17.91±0.26 mg respectively, where the flavonoid content in fruit extract with 44.69±0.30 mg/g of extract and the tannin content in bark extract with 62.37±1.75 mg/g of extract.
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Bhumi, G., & Savithramma, N. (2014). Screening of Pivotal medicinal plants for Qualitative and Quantitative phytochemical constituents. International Journal of Pharmacy and Pharmaceutical Sciences, 6(3), 63-65.
De Silva, G. O., Abeysundara, A. T., & Aponso, M. M. W. (2017). Extraction methods, qualitative and quantitative techniques for screening of phytochemicals from plants. American Journal of Essential Oils and Natural Products, 5(2), 29-32.
Harbone, J. B. (1973). Phytochemical methods: A Guide to Modern techniques of Plant analysis. London, UK: Chapman and Hill. https://doi.org/10.1007/978-94-009-5570-7.
Heinrich, M., Mah, J., & Amirkia, V. (2021). Alkaloids used as medicines: Structural Phytochemistry Meets Biodiversity -An Update and Forward Look. Molecules, 26(7), 1836. https://doi.org/10.3390/molecules26071836
Jayasree, S., Vijayakumar, N., Gomathi, K. S., & Helen, P. A. M. (2015). Study on Antimicrobial activity and Chemical profiling of Pamburus missionis (Wight) Swingle. International Journal of Pharmacy and Pharmaceutical Research, 2(3), 118-129.
Jayaweera, D. M. A. (1982). Medicinal Plants (Indigenous and Exotic) Used in Ceylon. Sri Lanka: National Science Council of Sri Lanka.
Kumar, V., Bulumulla, H. N. K., Wimalasiri, W. R., & Reisch, J. (1994). Coumarins and an indole alkaloid from Pamburus missionis. Phytochemistry, 36(4), 879-881. https://doi.org/10.1016/S0031-9422(00)90455-2
Lin, D., Xiao, M., Zhao, J., Li, Z., Xing, B., Li, X., Kong, M., Li, L., Zhang, Q., Liu, Y., Chen, H., Qin, W., Wu, H., & Chen, S. (2016). An Overview of Plant Phenolic Compounds and their importance in Human Nutrition and Mangement of Type 2 diabetes. Molecules, 21(10), 1374. https://doi.org/10.3390/molecules21101374
Matos, M. J., Santana, L., Uriarte, E., Abreu, O. A., Molina, E., & Yordi, E. G. (2015). Coumarins- an important class of Phytochemicals. In A. V. Rao & L. G. Rao (Eds.), Phytochemicals-Isolation, Characterisation and Role in Human health London, UK: IntechOpen Limited. https://doi.org/10.5772/59982
Mohan, P. R., & Savithramma, N. (2019). Screening of Phytochemicals and biosynthesis of silver nanoparticles from leaf, bark and fruits of medicinal tree species of Terminalia pallida Brandis an endemic to Seshachalam hill ranges. The Pharma Innovation Journal, 8(4), 408-416.
Okeke, C. U., & Elekwa, I. (2003). Phytochemical Study of the Extract of Gongronema latifolium Benth. Journal of Health and Visual Science, 5(1), 47-55.
Panda, M., Kumar, S., & Mahalik, G. (2019). An overview of medicinal plants of the family Rutaceae as a source of complementary therapeutics. Journal of Biodiversity and Conservation, 3(4), 13-17.
Parthasarathy, N., Selwyn, M. A., & Udayakumar, M. (2008). Tropical Dry Evergreen forests of Peninsular India: Ecology and Conservation Significance. Tropical Conservation Science, 1(2), 89-110. https://doi.org/10.1177/194008290800100203
Pavithra, P. S., Sreevidya, N., & Verma, R. S. (2009). Antibacterial activity and chemical composition of essential oil of Pamburus missionis. Journal of Ethnopharmacology, 124(1), 151-153. https://doi.org/10.1016/j.jep.2009.04.016
Peeriga, R., & Banoth, C. S. K. (2016). Pharmacognostical Investigation and Preliminary phytochemical screening of Leaves of Pamburus missionis S. Journal of Comprehensive Pharmacy, 3(3), 85-90.
Phuyal, N., Jha, P. K., Raturi, P. P., & Rajbhandary, S. (2020). Total Phenolic, Flavonoid Contents, and Antioxidant Activities of Fruit, Seed, and Bark Extracts of Zanthoxylum armatum DC. The Scientific World Journal, 2020, 8780704. https://doi.org/10.1155/2020/8780704
Sapkota, B., & Prakash, K. C., & Jain, V. (2020). Preliminary Phytochemical Screening and Quantitative Analysis of Citrus maxima (Brum.) Leaves Extract. International journal of Research in Pharmacology and Pharmacotherapeutics, 9(1), 100-106.
Savithramma, N., Rao, M. L., & Ankanna, S. (2012). Preliminary Phytochemical screening of some Important Medicinal plants. International Journal of Ayurvedic and Herbal Medicine, 2(1), 139-145.
Savithramma, N., Rao, M. L., & Prabha, B. (2011a). Phytochemical studies of Dysophylla myosuroides (Roth) Benth. In. Wall and Talinum cuneifolium (Vahl.) Willd. Research Journal of Phytochemistry, 5(3), 163-169.
Savithramma, N., Rao, M. L., & Suhrulatha, D. (2011b). Screening of Medicinal Plants for Secondary Metabolites. Middle-East Journal of Scientific Research, 8(3), 579-584.
Shaikh, J. R., & Patil, M. K. (2020). Qualitative tests for preliminary phytochemical screening: An overview. International Journal of Chemical Studies, 8(2), 603-608. https://doi.org/10.22271/chemi.2020.v8.i2i.8834
Sharma, T., Pandey, B., Shrestha, B. K., Koju, G. M., Thusa, R., & Karki, N. (2020). Phytochemical Screening of Medicinal Plants and Study of the Effect of Phytoconstituents in Seed Germination. Tribhuvan University Journal, 35(2), 1-11. https://doi.org/10.3126/tuj.v35i2.36183
Simpson, M. G. (2010). Diversity and classification of Flowering plants: Eudicots. In M. G. Simpson (Eds.), Plant Systematics (pp. 275-448) Cambridge, US: Academic Press. https://doi.org/10.1016/B978-0-12-374380-0.50008-7
Siva Prasad, K., Sujatha, K., Suhrulatha, D., & Savithramma, N. (2018). Screening of Nymphaea tetragona Georgi, a traditional taxon fro bioactive metabolites. Indian Journal of Applied Research, 8(11), 22-24.
Somasundaram, T. R. (1967). A Handbook on the Identification and Description of Trees, Shrubs and Some Important Herbs of the Forests of the Southern States: For the Use of the Southern Forest Rangers College. Coimbatore, Tamil Nadu: Southern Forest Rangers College.
Sornapudi, S. D., & Srivastava, M. (2022). Quantitative estimation of Phytochemicals in different leaf extracts. The Pharma Innovation Journal, 11(9), 125-128.
Swingle, W. T. (1916). Pamburus, a new genus related to citrus, from India. Journal of the Washington Academy of Sciences, 6(11), 335-338.
Wang, D., Wang, X.-H., Yu, X., Cao, F., Cai, X., Chen, P., Li, M., Feng, Y., Li, H., & Wang, X., (2021). Pharmacokinetics of Anthraquinones from Medicinal Plants. Frontiers in Pharmacology, 12, 638993. https://doi.org/10.3389/fphar.2021.638993
Yugandhar, P., & Savithramma, N. (2017). Spectroscopic and chromatographic exploration of different phytochemical and mineral contents from Syzygium alternifolim (Wt.) Walp. an endemic, endangered medicinal tree taxon. Journal of Applied Pharmaceutical Science, 7(1), 73-85. https://doi.org/10.7324/JAPS.2017.70110
Zhou, Y., Zheng, J., Li, Y., Xu, D.-P., Li, S., Chen, Y.-M., & Li, H.-B. (2016). Natural Polyphenols for Prevention and Treatment of Cancer. Nutrients, 8(8), 515. https://doi.org/10.3390/nu8080515
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