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TY - JOUR
AU - V, Puttalingamma
AU - R, Shylaja
AU - V, Batra H
AU - S, Bawa A
PY - 2012/07/05
Y2 - 2025/09/23
TI - A novel multiplex PCR system for the detection of virulence associated genes of E. coli O157:H7 from food system
JF - Recent Research in Science and Technology
JA - RRST
VL - 4
IS - 5
SE - Articles
DO -
UR - https://www.updatepublishing.com/journal/index.php/rrst/article/view/897
SP -
AB - <p class="MsoNormal" style="text-align: justify;">In view of the importance E. coli serotype 0157:H7 in human diseases, an easy and quick system is desirable to detect toxin producing strains. In this report, we describe standardization of a novel multiplex Polymerase Chain Reaction (mPCR) assay for simultaneous detection of four important genes associated with the organism E. coli 0157:H7 viz., stx 1, stx 2, eae A, hly A along with an internal amplification control (IAC). The mPCR method developed in the present study is sensitive enough to detect cells as low as 103 CFU ml-1 or g-1 of the food samples. The E. coli 0157:H7 strains having been identified to contain the gene in the mPCR were unequivocally detected positive for the serological and conventional culture method. As E. coli 0157:H7 is qualified as biowarfare agent; this mPCR system is of immense help in detecting them during emergencies of biological war and suspected outbreaks. </p>
ER -