Warning: ini_set(): A session is active. You cannot change the session module's ini settings at this time in /home/updatepublishing/public_html/journal/lib/pkp/classes/session/SessionManager.inc.php on line 69

Warning: Cannot modify header information - headers already sent by (output started at /home/updatepublishing/public_html/journal/lib/pkp/classes/session/SessionManager.inc.php:69) in /home/updatepublishing/public_html/journal/plugins/generic/citationStyleLanguage/CitationStyleLanguagePlugin.inc.php on line 478

Warning: Cannot modify header information - headers already sent by (output started at /home/updatepublishing/public_html/journal/lib/pkp/classes/session/SessionManager.inc.php:69) in /home/updatepublishing/public_html/journal/plugins/generic/citationStyleLanguage/CitationStyleLanguagePlugin.inc.php on line 479
TY - JOUR AU - Bawa, Alhassan AU - Isaac, Addai Kwahene AU - Abdulai, Mashark Seidu PY - 2015/10/19 Y2 - 2025/09/23 TI - SSR markers as tools for screening genotypes of maize (Zea mays L.) for tolerance to drought and Striga hermonthica (Del.) Benth in the Northern Guinea Savanna Zone of Ghana JF - Research in Plant Biology JA - RIPB VL - 5 IS - 5 SE - Articles DO - UR - https://www.updatepublishing.com/journal/index.php/ripb/article/view/2648 SP - AB - <p>ABSTACT</p> <p>Resistant or tolerant crop cultivars provide the most economical, practical and sustainable method of control of Striga and drought stresses. However, the development of resistant/tolerant maize cultivars is hampered by the complexity of the environment, the host/parasite interactions, and lack of reliable screening method. The invention of molecular markers to tag gene(s) that confer important traits offer new hope for Striga and drought control. A study was conducted at the Biotechnology laboratory of the Savanna Agricultural Research Institute in Nyankpala, Northern Ghana, to screen six parent and thirty F₁ progenies of maize for tolerance to drought and <em>Striga hermonthica</em> using 17 microsatellites. Genomic DNA was extracted with the CTAB method and PCR was performed based on the common method for microsatellite markers. PCR products were separated using 6% polyacrylamide denaturing gel. Frequencies were run to determine the genotypes that produce bands across all or most microsatellite primers.</p> <p>The results indicated that the F₁hybrids IWD x TAIS03, IWD x GUMA03, DT x KOBN03, TAIS03 x IWD, TAIS03 x DT, TAIS03 x SISF03, SISF03 x TAIS03, SISF03 x GUMA03, GUMA03 x IWD, GUMA03 x DT and GUMA03 x KOBN03  contained the quantitative traits responsible for both drought and <em>Striga hermonthica</em> tolerance. The parental lines DT-STR-W-C2, TAIS03 and IDW-C3-SYN-F2, and F₁ hybrid populations IWD x TAIS03, IWD x GUMA03, DT x KOBN03, TAIS03 x IWD, TAIS03 x DT, TAIS03 x SISF03, TAIS03 x KOBN03, SISF03 x DT, SISF03 x TAIS03, SISF03 x GUMA03, GUMA03 x IWD, GUMA03 x DT, GUMA03 x SISF03, GUMA03 x KOBN03 and KOBN03 x IWD contained the quantitative traits responsible for <em>Striga hermonthica</em> tolerance. The study revealed that the parent populations IDW-C3-SYN-F2, TAIS03, GUMA03-OB and KOBN03-OB, and F₁ hybrid populations IWD x TAIS03, IWD x GUMA03, IWD x KOBN03, DT x TAIS03, DT x SISF03, DT x KOBN03, TAIS03 x IWD, TAIS03 x DT, TAIS03 x SISF03, SISF03 x TAIS03, SISF03 x GUMA03, SISF03 x KOBN03, GUMA03 x IWD, GUMA03 x DT and GUMA03 x KOBN03 showed bands across nine drought-linked SSR markers, and therefore, contain the quantitative traits responsible for drought tolerance.</p> <p>The study led to identification of informative SSR markers, such as Phi074, Nc005, Phi015, Phi061 and Phi055, which significantly contributed to the differentiation of Striga tolerant and susceptible genotypes. Identification of informative SSR markers (P-umc1542, P-bnlg1429, P-umc1566, P-umc2189, P-umc2225, P-umc2226, P-bnlg1014, P-bnlg1124 and P-umc1292) which significantly contributed to the differentiation of drought tolerant and susceptible genotypes was also made.</p> ER -