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TY  - JOUR
AU  - Joseph, Nisha
AU  - Siril, E.A.
PY  - 2013/02/08
Y2  - 2025/09/23
TI  - Multiplication of Annatto (Bixa orellana L.) using cotyledons and leaf explants
JF  - Research in Plant Biology
JA  - RIPB
VL  - 3
IS  - 1
SE  - Articles
DO  - 
UR  - https://www.updatepublishing.com/journal/index.php/ripb/article/view/2526
SP  - 
AB  - Annatto (&lt;em&gt;Bixa orellana&lt;/em&gt; L.) is an important natural dye yielding plant. To improve theregeneration capacity of annatto, the effects of explants size, plant growth regulators, lightregime and leaf position were evaluated using cotyledons and leaf explants derived fromseedlings. Explants having entire lamina with petiole showed maximum response. Thecotyledonary explants produced highest regeneration frequency (78.1%) and maximumnumber of shoots (12.4) on Murashige and Skoog medium supplemented with 20 ÃŽÂ¼M benzyladenine (BA). Leaf explants on MS medium containing 20 ÃŽÂ¼M BA gave 67% regenerationfrequency and 11.3 new shoots per explant. A photoperiod of 16/8 h (light/dark) was foundto be superior to continuous dark or light. Position of leaf on the seedling had significantrole in organogenic response and is found decreasing from the base to shoot apex. Theregenerated shoots were rooted on MS medium supplemented with 10 ÃŽÂ¼M IBA. The rootedplantlets were transferred initially in the growth chamber and then moved to a green house.The present protocol can be utilized for the mass multiplication and also for effective genetictransformation aimed at the improvement of annatto.
ER  -