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TY - JOUR
AU - Krapp, Florian
AU - dos Santos Cruz, Geyner Alves
AU - Wöhrmann, Tina
AU - Benko-Iseppon, Ana Maria
AU - Weising, Kurt
PY - 2013/04/08
Y2 - 2025/09/23
TI - A set of variable plastid SSR markers for the genus Cryptanthus (Bromeliaceae)
JF - Research in Plant Biology
JA - RIPB
VL - 3
IS - 2
SE - Short Communications
DO -
UR - https://www.updatepublishing.com/journal/index.php/ripb/article/view/2521
SP -
AB - The genus <em>Cryptanthus</em> (Bromeliaceae) is endemic to Brazil. Many of its currentlyrecognized 66 species are narrow endemics that are threatened by habitat destruction.Molecular markers are needed to evaluate the extent and distribution of genetic diversityin rare <em>Cryptanthus </em>species, which would be a prerequisite for taking appropriateconservation measures. Here we describe the development of plastid microsatellitemarkers (cpSSRs) for <em>Cryptanthus</em>. PCR primers specific for 34 cpSSR loci in <em>Dyckiamarnier-lapostollei</em> were initially tested for their functionality in <em>Cryptanthus schwackeanus</em>. PCR was successful for 29 loci, and 13 loci were shown to harbour extended stretches of mononucleotide repeats. Seven loci were further characterized bygenotyping <em>Cryptanthus </em>samples at the level of populations and species, and six lociproved to be polymorphic among 30 individuals of each of the two endangered species <em>C. schwackeanus</em> and <em>C. warren-loosei</em>, respectively. All primers cross-amplified in other genera from three subfamilies of Bromeliaceae.
ER -