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TY - JOUR AU - Chakravarthy, Vajhala S.K. PY - 2013/10/21 Y2 - 2025/09/23 TI - Rapid production of multiple shoots from cotyledonary node explants of an elite cotton (Gossypium hirsutum L.) variety JF - Research in Plant Biology JA - RIPB VL - 3 IS - 5 SE - Articles DO - UR - https://www.updatepublishing.com/journal/index.php/ripb/article/view/2495 SP - AB - <em>In vitro</em> sprouting and simultaneous elongation of multiple shoots were achievedby culturing cotyledonary node explants of an elite cotton (<em>Gossypium hirsutum L.</em>)variety (NC-601), on Murashige and Skoog basal medium containing N6-Benzylaminopurine, 6- Furfurylaminopurine and Thidiazuron in combination with 1-Naphthaleneacetic acid. A combination of N6-Benzylaminopurine (1.5mg/l) and 1-Naphthaleneacetic acid (0.5mg/l) was found most effective for producing maximumnumber (10.31±0.22) of multiple shoots per explant. Although the explants of differentsets of seedlings could produce multiple shoots, the explants derived from the 15-day oldseedlings show maximum response compared to 5- and 10-day old nodal explants.Furthermore, maximum frequency (82.6%) of rooting was observed in the elongatedshoots when cultured on the half-strength MS medium containing Indole-3-butyric acid.Regenerated plants could survive upto 95 to 100% under the glass house conditions.Following this procedure, plants were obtained within a period of 12 to 15 weeks. Normalflowering and boll formation were observed in regenerated plants similar to that ofcontrol plants obtained through seed germination. ER -