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TY - JOUR
AU - Bansal, Shikha
AU - Bharati, Asha Jyoti
AU - Bansal, Yogendra Kumar
PY - 2013/12/20
Y2 - 2025/09/23
TI - Isolation and identification of flavonoid from in vitro and natural grown Nyctanthes arbor-tristis L. (Harsingar) - a medicinally important plant
JF - Research in Plant Biology
JA - RIPB
VL - 3
IS - 6
SE - Articles
DO -
UR - https://www.updatepublishing.com/journal/index.php/ripb/article/view/2491
SP -
AB - A rapid propagation protocol was established and optimized<em> in vitro</em> for <em>Nyctanthesarbor-tristis</em> L. a small ornamental tree with medicinal and aromatic properties. Nodalexplants were cultured on Murashige and Skoog (MS) medium supplemented with variousplant growth regulators: BAP and Kn in combination. Maximum frequency of shoot initiation(FSI) (1-2 wks) and mean shoot number (MSN) 3.02±0.437 were obtained on BAP + Kn (1.0 +0.1), whereas high mean shoot length (MSL) 2.9±0.356 was observed on BAP + Kn (2.0 + 0.5)Elongated microshoots (3-4 wks) were successfully rooted and regenerated plants weresimultaneously hardened and acclimatized. An approximately 91% survival rate wasrecorded. Natural leaf, stem, <em>in vitro</em> leaf and stem were subsequently extracted in 80%methanol and were subjected to thin layer chromatography along with the standard.
ER -