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@article{Adenegan-Alakinde_2023, title={Taxonomic significance of leaf architecture in the genus Basella Linn. in Nigeria}, volume={13}, url={https://www.updatepublishing.com/journal/index.php/ripb/article/view/8576}, DOI={10.25081/ripb.2023.v13.8576}, abstractNote={<p>This study seeks to use the leaf architecture as a taxonomic tool for members of the genus <em>Basella </em>Linn. in Nigeria, namely, <em>B. rubra </em>Linn., <em>B. alba </em>Linn., <em>B. cordifolia </em>Lamk. and a form, <em>B. alba </em>round because the taxonomy of the genus <em>Basella </em>Linn. is not clear and there is no information on the leaf architecture of the genus. For studies of the venation pattern of the leaves, sizeable portions were taken from the standard median portion of mature leaves. Cleared leaves were stored in 50% alcohol for anatomical studies. Leaves were stained in Safranin O for 3 minutes and counterstained in Alcian blue and then were rinsed in water to remove excess stain. Stained cleared leaves were treated in serial grades of alcohol for differentiation and dehydration. Stained materials were mounted in dilute glycerol for microscopic examination. Photomicrographs were taken with the aid of 3013 ACCU-SCOPE Trinocular Microscope with Digital Camera. The areolar area was calculated from the length and breadth of the areoles measured using a micrometer inserted into the microscope eyepiece. <em>Basella </em>species have common generic features. Areoles are closed in <em>B. rubra </em>but others have veinlet endings. The area of areole is diagnostic because in <em>B. rubra </em>the area is 305,370±1808.91 μm<sup>2</sup>; <em>B. alba </em>is 501,796.75±3217.38 μm<sup>2</sup>, <em>B. cordifolia</em>’s area of areole is 396,394.75±2670.67 μm<sup>2</sup> while that of <em>B. alba </em>round is 540,058.75±3702.28 μm<sup>2</sup>. The arrangement of druses in the areoles is also diagnostic.</p>}, journal={Research in Plant Biology}, author={Adenegan-Alakinde, Taiwo Ayomipo}, year={2023}, month={Oct.}, pages={25–28} }