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@article{Robinson_Srivardhini_Sasikumar_2011, title={Somatic embryogenesis and plant regeneration from cotyledon tissue of Arachis hypogaea L.}, volume={1}, url={https://www.updatepublishing.com/journal/index.php/ripb/article/view/2579}, abstractNote={Tissue culture techniques are useful for ex situ conservation of rare, endemic orthreatened plant species. This report describes a protocol for somatic embryogenesis ofpeanut (Arachis hypogaea L.) through cotyledon explants. Only 10% of the explants werecontaminated by using this material to start the in vitro culture. The highest frequency(35.4%) of embryogenic calli induction was observed on MS medium supplemented with0.50 mg/l KIN in combination with 0.10 mg/l 2,4,-D. the percentage response for embryogenicproliferation increased in the medium supplemented with GA3(0.20 mg/l) and 1.0 mg/l BA.The regenerated embryos were successfully transferred to the embryo development mediumand transferred to the trays after the development of secondary and tertiary rootdevelopment.}, number={3}, journal={Research in Plant Biology}, author={Robinson, J. Philip and Srivardhini, S. and Sasikumar, G.}, year={2011}, month={Aug.} }