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@article{Bansal_Bharati_Bansal_2013, title={Isolation and identification of flavonoid from in vitro and natural grown Nyctanthes arbor-tristis L. (Harsingar) - a medicinally important plant}, volume={3}, url={https://www.updatepublishing.com/journal/index.php/ripb/article/view/2491}, abstractNote={A rapid propagation protocol was established and optimized<em> in vitro</em> for <em>Nyctanthesarbor-tristis</em> L. a small ornamental tree with medicinal and aromatic properties. Nodalexplants were cultured on Murashige and Skoog (MS) medium supplemented with variousplant growth regulators: BAP and Kn in combination. Maximum frequency of shoot initiation(FSI) (1-2 wks) and mean shoot number (MSN) 3.02±0.437 were obtained on BAP + Kn (1.0 +0.1), whereas high mean shoot length (MSL) 2.9±0.356 was observed on BAP + Kn (2.0 + 0.5)Elongated microshoots (3-4 wks) were successfully rooted and regenerated plants weresimultaneously hardened and acclimatized. An approximately 91% survival rate wasrecorded. Natural leaf, stem, <em>in vitro</em> leaf and stem were subsequently extracted in 80%methanol and were subjected to thin layer chromatography along with the standard.}, number={6}, journal={Research in Plant Biology}, author={Bansal, Shikha and Bharati, Asha Jyoti and Bansal, Yogendra Kumar}, year={2013}, month={Dec.} }