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@article{Gothandam_2011, title={Characterization of cathepsin B-like gene from rice}, volume={1}, url={https://www.updatepublishing.com/journal/index.php/ripb/article/view/2486}, abstractNote={In this study, a rice cDNA clone encoding a putative cysteine protease withsequence homology to mammalian cathepsin B has been isolated and characterized.Sequence analysis revealed that the gene designated <em>OsCatB</em> encodes a cysteine proteasethat belongs to the papain family without the consensus motif, ERFNIN. The resultsuggests that the <em>OsCatB</em> is the cathepsin B-like protein in rice. Among the cathepsin Blikeproteins in plants, the highest homology was occurred to the sequence of the barleycathepsin B. DNA blot analysis showed that the <em>OsCatB</em> gene belongs to a multiple genefamily in the rice genome. Expression analysis of the <em>OsCatB</em> revealed that the gene isexpressed in all organs of rice including the germinating seed. To elucidate <em>OsCatB</em> function, we generated knockout mutants by RNAi system. Microscopic analysis revealedthat knockout of the <em>OsCatB</em> gene caused an excessive cell death and suggested that thegene functions as an anti-cell-death component.}, number={1}, journal={Research in Plant Biology}, author={Gothandam, K.M.}, year={2011}, month={Mar.} }