Establishment of an Efficient Protocol for in Vitro Callus Induction and Regeneration System Using Mature Embryo in Elite Rice (Oryza sativa L.) Cultivars

Abstract

The present investigation was undertaken to determine a suitable media compositions for callus induction and plant regeneration of rice (Oryza sativa L.) through somatic embryogenesis. Murashige and Skoog (MS) media composition and phytohormones (i.e. auxin; 2, 4-D, IAA, IBA and cytokinin; BAP, KIN) were tested for high callus induction and plant regeneration. Mature seeds of six rice cultivars namely BRRI dhan28, BRRI dhan29, BRRI dhan30, BRRI dhan34, BRRI dhan56 and BRRI dhan57 were used for the experimentation. 2.0 mg/l of 2,4-D + 0.5 mg/l of NAA were found suitable inducing high amount of calli and 2.0 mg/l of BAP+ 1.0 mg/l of NAA + 1.5 mg/l of KIN were found most effective for plant regeneration. Among cultivars for callus induction, BRRI dhan29 shows the highest percentage and BRRI dhan30 less and remaining cultivars (BRRI dha28, BRRI dhan34, BRRI dhan56 and BRRI dhan57) show moderate results in the experiments. Among cultivars for regeneration, BRRI dhan29 shows the highest percentage and BRRI dhan30 less and remaining cultivars (BRRI dha28, BRRI dhan34, BRRI dhan56 and BRRI dhan57) show moderate results. The investigation will provide valuable information for Agrobacterium mediated genetic transformation.