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TY  - JOUR
AU  - Jeba Malar T, Renisheya Joy
AU  - M, Johnson
PY  - 2016/01/03
Y2  - 2025/09/23
TI  - Biosynthesis of Silver nanoparticles using Plumbago zeylanica Leaves extracts and their bio-efficacy
JF  - Research in Pharmacy
JA  - RIP
VL  - 5
IS  - 0
SE  - Articles
DO  - 
UR  - https://www.updatepublishing.com/journal/index.php/rip/article/view/2941
SP  - 
AB  - &lt;p class=&quot;Default&quot;&gt;&lt;strong&gt;The present study was intended to optimize the protocol for the synthesis of silver nano particles of &lt;em&gt;Plumbago zeylanica&lt;/em&gt; Linn. leaves extracts and evaluate their bioefficacy against six different human pathogens. The aqueous silver ions exposed to the leaves extracts and the silver nanoparticle synthesis was established by the change of colour of leaves extracts from greenish to reddish brown. The&lt;em&gt; in vitro&lt;/em&gt; synthesised silver nanopeptides were further confirmed by using UV-Vis spectroscopy and examined their antibacterial activity against &lt;em&gt;S. aureus&lt;/em&gt;,&lt;em&gt; B. subtilis&lt;/em&gt;,&lt;em&gt; S. pyogenes &lt;/em&gt;(Gram positive), &lt;em&gt;K. pneumoniae&lt;/em&gt;,&lt;em&gt; M. morganii&lt;/em&gt;,&lt;em&gt; P. aeruginosa&lt;/em&gt; (Gram negative).&lt;/strong&gt;&lt;strong&gt; &lt;/strong&gt;&lt;strong&gt;Â To find the inhibition concentrations, the antibacterial activity was performed by agar well diffusion method with five different concentrations (10 â€“ 50 Âµg/ml) against six different pathogens viz., &lt;em&gt;S. aureus&lt;/em&gt;,&lt;em&gt; B. subtilis&lt;/em&gt;,&lt;em&gt; S. pyogenes &lt;/em&gt;(Gram positive), &lt;em&gt;K. pneumoniae&lt;/em&gt;,&lt;em&gt; M. morganii&lt;/em&gt;,&lt;em&gt; P. aeruginosa&lt;/em&gt; (Gram negative).&lt;/strong&gt;&lt;strong&gt; &lt;/strong&gt;&lt;strong&gt;Â The zone of inhibition was directly consonance with the concentrations of &lt;em&gt;P. zeylanica&lt;/em&gt; SNPS. 50 Âµl of &lt;em&gt;P. zeylanica&lt;/em&gt; SNPs showed maximum zone of inhibition (&lt;/strong&gt;&lt;strong&gt;25Â±0.5 mm) &lt;/strong&gt;&lt;strong&gt;against &lt;em&gt;K. pneumoniae&lt;/em&gt;, next to that, &lt;/strong&gt;&lt;strong&gt;23Â±0.5 mm zone of inhibition was observed against &lt;em&gt;M. morganii&lt;/em&gt;&lt;/strong&gt;&lt;strong&gt; and minimum zone of inhibition (&lt;/strong&gt;&lt;strong&gt;9 Â± 0.3 mm) &lt;/strong&gt;&lt;strong&gt;was obtained aginst &lt;/strong&gt;&lt;strong&gt;&lt;em&gt;S. pyogenes&lt;/em&gt;&lt;/strong&gt;&lt;strong&gt;. The results revealed that the AgNPâ€™s extract of &lt;em&gt;P. zeylanica&lt;/em&gt; showed higher zone of inhibition against Gram negative bacteria than the Gram positive bacteria.&lt;/strong&gt;&lt;/p&gt;
ER  -