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TY - JOUR
AU - Vijayan, Murali Vijayan
AU - Dharmalingam Jothinathan, Mukesh Kumar
AU - Murugesan, Suresh Kumar
AU - Rangasamy, Nandakumar
AU - Sankaran, Ganesan
AU - Sathasivam, Vinoth
AU - M, Prasanth
AU - T, Shanthini
PY - 2015/12/28
Y2 - 2025/09/23
TI - Microbial Production of Amylase from Cassava Waste
JF - Research in Pharmacy
JA - RIP
VL - 5
IS - 0
SE - Articles
DO -
UR - https://www.updatepublishing.com/journal/index.php/rip/article/view/2931
SP -
AB - <p><strong><em>Bacterium mura </em></strong><strong>was isolated from cassava waste, (Tamil Nadu, India) for the production of extracellular amylase. On screening for amylase producing bacteria, 5 isolates showed positive results, of which <em>Bacterium mura </em>showed best amylase activity. The optimal conditions for the amylase activity were found at pH 6.0 (39 U/ml) and at temperature 37°C. Amylase activity was found to be higher when lactose (31 U/ml), casein, barley (42 U/ml) and SDS (32 U/ml) were used as the carbon source, nitrogen source, agro waste source and as additives respectively. The enzyme was partially purified by dialysis and the molecular mass was found to be 65kDa by SDS-PAGE. The partially purified and crude amylase was confirmed by zymogram. The partially purified amylase was used in bread making, which improved the softening of the bread and was used as a de-sizing agent.</strong></p>
ER -