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@article{Vijayan_Dharmalingam Jothinathan_Murugesan_Rangasamy_Sankaran_Sathasivam_M_T_2015, title={Microbial Production of Amylase from Cassava Waste}, volume={5}, url={https://www.updatepublishing.com/journal/index.php/rip/article/view/2931}, abstractNote={<p><strong><em>Bacterium mura </em></strong><strong>was isolated from cassava waste, (Tamil Nadu, India) for the production of extracellular amylase. On screening for amylase producing bacteria, 5 isolates showed positive results, of which <em>Bacterium mura </em>showed best amylase activity. The optimal conditions for the amylase activity were found at pH 6.0 (39 U/ml) and at temperature 37°C. Amylase activity was found to be higher when lactose (31 U/ml), casein, barley (42 U/ml) and SDS (32 U/ml) were used as the carbon source, nitrogen source, agro waste source and as additives respectively. The enzyme was partially purified by dialysis and the molecular mass was found to be 65kDa by SDS-PAGE. The partially purified and crude amylase was confirmed by zymogram. The partially purified amylase was used in bread making, which improved the softening of the bread and was used as a de-sizing agent.</strong></p>}, journal={Research in Pharmacy}, author={Vijayan, Murali Vijayan and Dharmalingam Jothinathan, Mukesh Kumar and Murugesan, Suresh Kumar and Rangasamy, Nandakumar and Sankaran, Ganesan and Sathasivam, Vinoth and M, Prasanth and T, Shanthini}, year={2015}, month={Dec.} }