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TY - JOUR AU - Nathalie, Mbega Sanama AU - Bertrand, Mboussi Serge AU - Christian, Gbaporo Gbaporo Fabrice AU - Landry, Dida Lontsi Sylvère AU - Patrice, Ngatsi Zemko AU - Ebenezer, Foka Tatiekam AU - Landry, Dongmo Voukeng Thiery AU - Zachée, Ambang PY - 2024/12/31 Y2 - 2025/09/23 TI - Phytochemical characterization of sub-fractions of Thevetia peruviana, Azadirachta indica and their antifungal efficacy on Pythium myriotylum, the causal agent of cocoyam root rot disease (Xanthosoma sagittifolium L. Schott) JF - Research in Biotechnology JA - RIB VL - 15 IS - SE - Articles DO - 10.25081/rib.2024.v15.9097 UR - https://www.updatepublishing.com/journal/index.php/rib/article/view/9097 SP - 26-36 AB - <p><em>Pythium myriotylum </em>is a telluric Oomycete, the causal agent of cocoyam root rot disease. In Cameroon, it can cause crop losses of up to 100% without sanitary measures. The aim of this study was to evaluate the antifungal efficacy of bioactive compounds from <em>Thevetia peruviana </em>(yellow oleander) and <em>Azadirachta indica </em>(neem) sub-fractions obtained by GC/MS against <em>P. myriotylum. </em>The antifungal activities of crude extracts (2%; 1%; 0.5% (m/v)) of <em>T. peruviana </em>and <em>A. indica </em>(2% and 0.5% (m/v)) in water, methanol and acetone were evaluated. The sub-fractions resulting from the roughing of the most active fractions by VLC (Vacum Liquid column) were characterized by GC/MS and their minimum inhibitory concentrations (MIC) were determined, as well as their mode of action on <em>P. myriotylum</em>. The results obtained show that GC/MS analysis identified many compounds with antifungal activities such as Oxime-, methoxy-phenyl, Hexadecanoic acid, methyl ester; 9,12-Octadecadienoic acid, methyl ester, (E,E); 7-Octadecenoic acid, methyl ester; 9-Octadecenoic acid, methyl ester, (E); Methyl stearate, Phenol, 2,2’-methylenebis[6-(1,1-dimethylethyl)-4-methyl. All fractions derived from crude extracts significantly reduced the growth of <em>P. myriotylum </em>compared with the negative control, with the highest inhibition rates obtained with the aqueous ethyl acetate phase fractions (87.77% and 100% for <em>T. peruviana </em>and <em>A. indica</em>, respectively). The most active sub-fractions F1 of VLC2 and F4 of VLC1 inhibited the pathogen’s protein synthesis. The lowest MICs were obtained with sub-fractions F1 of <em>A. indica </em>and F4 of <em>T. peruviana </em>(0.166% and 0.0837%). <em>T. peruviana </em>and <em>A. indica </em>can be considered as potential substitutes for chemical control.</p> ER -