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TY - JOUR AU - Torres, Gustavo Rubens Castro AU - Houllou, Laureen Michelle AU - de Souza, Robson Antônio PY - 2016/08/08 Y2 - 2025/09/23 TI - Control of contaminants during introduction and establishment of Bambusa vulgaris in vitro JF - Research in Biotechnology JA - RIB VL - 7 IS - 0 SE - Research Articles DO - 10.19071/rib.2016.v7.3056 UR - https://www.updatepublishing.com/journal/index.php/rib/article/view/3056 SP - AB - The aim of this work was to test techniques to reduce microbial contamination in the phases of introduction and establishment of the <em>in vitro</em> cultivation of <em>Bambusa vulgaris</em> through two experiments. The first experiment was carried out in a completely randomized design using a factorial arrangement (pre-treatment of nodal segments using or not a solution of Derosal 500 SC^® and Chloramphenicol × culture medium with half or full concentration of salts × culture medium with presence or absence of sucrose × culture medium with presence or absence of Plant Preservative Mixture^TM). In a second experiment, carried out in a completely randomized design, the effect of different fungicides associated to Chloramphenicol in a liquid culture medium was tested. It was possible to verify that the isolated effects of the pre-treatment by immersion of the nodal segments in a solution of 4 mL L^-1of Derosal 500 SC^®and 200 mg L^-1of Chloramphenicol for 30 minutes and explants placed in a sucrose-free medium reduced fungal contamination. In the second experiment, the treatment that reduced fungal contamination corresponded to explants placed for seven days in a liquid medium with half the concentration of salts, sucrose-free, with 2 mL L^-1 of Plant Preservative Mixture^TMand with 4 mL L^-1of ^ÂDerosal 500 SC^® and 200 mg L^-1of Chloramphenicol. ER -