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TY  - JOUR
AU  - Khan, Shahana
AU  - Shah, Rehana Anjum
PY  - 2016/02/06
Y2  - 2025/09/23
TI  - Assessment of genetic diversity among India Ginseng, Withania somnifera (L) Dunal using RAPD and ISSR markers
JF  - Research in Biotechnology
JA  - RIB
VL  - 7
IS  - 0
SE  - Research Articles
DO  - 10.19071/rib.2016.v7.2818
UR  - https://www.updatepublishing.com/journal/index.php/rib/article/view/2818
SP  - 
AB  - &lt;em&gt;Withania somnifera&lt;/em&gt; is commonly known as Indian Ginseng or Ashwagandha which is listed highly medicinal plant in Ayurveda for its wide range of medicinal use. Genetic diversity among 16 genotypes of &lt;em&gt;Withania somnifera&lt;/em&gt; collected from four different regions viz. Lucknow, Nimuch, Karnataka and Mumbai was studied using RAPD and ISSR techniques. The ISSR and RAPD together produced 89 bands across 16 genotypes of &lt;em&gt;W. somnifera &lt;/em&gt;of which 39 were polymorphic showing 47.89% polymorphism. Over 99% of the RAPD and ISSR fragments were reproducible in the present experiment. There was 90% uniformity within the population. The neighbor joining tree also confirmed a clear grouping and differentiation based on populations of origin i.e. samples were grouped on the basis of their regions of origin. The dendrogram reveals that outgroup of &lt;em&gt;W. somnifera &lt;/em&gt;Karnataka origin are evolutionarily related to &lt;em&gt;W. somnifera &lt;/em&gt;Nimuch group varieties and &lt;em&gt;W. somnifera &lt;/em&gt;Lucknow and Mumbai group varieties are evolutionarily closely related. Within population analysis of Lucknow and Nimuch location showed two sub groups of two lines each showing slight diversity. Plants from Karnataka and Mumbai region show less diversity within population.
ER  -