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TY - JOUR AU - Devaraj, Natarajan AU - Ragavendran, C AU - Kamalanathan, D PY - 2014/03/07 Y2 - 2025/09/23 TI - A Rapid Micropropagation of nodal explants of Eclipta alba (L.); A Multipurpose Medicinal Herb JF - Research in Biotechnology JA - RIB VL - 5 IS - 2 SE - Research Articles DO - UR - https://www.updatepublishing.com/journal/index.php/rib/article/view/2451 SP - AB - <p><strong>An efficient <em>in vitro </em>regeneration protocol was developed for medicinally important plant <em>Eclipta alba</em>.<em> </em>Successful regeneration and multiplication of nodal explants of <em>E. alba</em> were obtained in cytokinin enriched B5 medium. Several cytokinins [6-benzylaminopurine (BAP), kinetin (KIN), thidiazuron (TDZ), gibberellic acid (GA₃) and spermidine] were supplemented alone and its combinations for obtaining better results. The best growth frequency response was achieved in the combinations of 1.0 BAP + 0.3 KIN + 1.5 GA₃ (mg/L) concentration (7.4 ± 0.9 cm shoot length &amp; 100 % regeneration). Better roots were developed in half-strength B5 medium along with IBA (1.0 mg/L) hormone and exhibits maximum root length (7.0 ± 0.8cm) along with multiple roots (8.8 ± 0.8) at 92 %. The well-developed Plantlets were successfully acclimatized to plastic-cups containing autoclaved sand and garden soil (1:1) and kept undisturbed with plastic cover for maintaining the humidity. The plantlets were watered regularly and maintained at green house. </strong></p> ER -