Warning: ini_set(): A session is active. You cannot change the session module's ini settings at this time in /home/updatepublishing/public_html/journal/lib/pkp/classes/session/SessionManager.inc.php on line 69

Warning: Cannot modify header information - headers already sent by (output started at /home/updatepublishing/public_html/journal/lib/pkp/classes/session/SessionManager.inc.php:69) in /home/updatepublishing/public_html/journal/plugins/generic/citationStyleLanguage/CitationStyleLanguagePlugin.inc.php on line 478

Warning: Cannot modify header information - headers already sent by (output started at /home/updatepublishing/public_html/journal/lib/pkp/classes/session/SessionManager.inc.php:69) in /home/updatepublishing/public_html/journal/plugins/generic/citationStyleLanguage/CitationStyleLanguagePlugin.inc.php on line 479
TY - JOUR AU - C, Chandrika AU - C, Vijayashree AU - S, Granthali AU - S, Rajath AU - G S, Nagananda AU - S, Sundara Rajan PY - 2013/08/20 Y2 - 2025/09/23 TI - A comparative kinetic study on β-amylase and its antioxidant property in germinated and non germinated seeds of Glycine max. L JF - Research in Biotechnology JA - RIB VL - 4 IS - 4 SE - Research Articles DO - UR - https://www.updatepublishing.com/journal/index.php/rib/article/view/2436 SP - AB - <p>Enzyme activity plays a key role in each stage of plant development starting from the initial seed germination responses. A comparative analysis of beta amylase activity was performed, isolated from germinated and non germinated <em>Glycine max</em> seeds, which was partially purified by dialysis. The optimum pH and temperature for germinated seeds was found to be 4.5 and 37⁰C and that for non germinated seeds were 5.5 and 75⁰C respectively. MnCl₂ and CoCl₂ exhibited marked activating effect on the enzyme, while HgCl₂ was a potent inhibitor for both seeds. The K_m and Vmax value for β-amylase with soluble starch as substrate was found to be 3.03mg/ml and 6.6micromol/min/ml for germinated seed and for non germinated seeds it was found to be 5mg/m and 10micromole/min/ml respectively. The molecular weight of partially purified enzyme was 57±1kDa on SDS PAGE with 1.918 fold purification and 27.98% yield for germinated seeds and for non germinated seeds the purification fold was 1.507 with 26.65% yield. The enzyme showed highest amount of total antioxidant activity of 304µg/ml in partially purified germinated seed. For ABTS free radical scavenging activity, partially purified enzyme from germinated seed showed the highest of 76.09% activity with an IC₅₀ value of 58.20µg/ml<strong>. </strong>Germinated seeds have highest enzymatic activity which can be considered for several industrial purposes.</p> <p><strong></strong></p> ER -