Warning: ini_set(): A session is active. You cannot change the session module's ini settings at this time in /home/updatepublishing/public_html/journal/lib/pkp/classes/session/SessionManager.inc.php on line 69

Warning: Cannot modify header information - headers already sent by (output started at /home/updatepublishing/public_html/journal/lib/pkp/classes/session/SessionManager.inc.php:69) in /home/updatepublishing/public_html/journal/plugins/generic/citationStyleLanguage/CitationStyleLanguagePlugin.inc.php on line 478

Warning: Cannot modify header information - headers already sent by (output started at /home/updatepublishing/public_html/journal/lib/pkp/classes/session/SessionManager.inc.php:69) in /home/updatepublishing/public_html/journal/plugins/generic/citationStyleLanguage/CitationStyleLanguagePlugin.inc.php on line 479
TY - JOUR AU - G.S, Nagananda AU - S, Rajath AU - Mathew, Ruby. K. AU - Rajan, S. Sundara PY - 2012/09/03 Y2 - 2025/09/23 TI - Effect of adjuvants and nitrogen sources on in vitro shoot regeneration and clonal propagation of medicinally important plant Eryngium foetidum L JF - Research in Biotechnology JA - RIB VL - 3 IS - 5 SE - Short Communications DO - UR - https://www.updatepublishing.com/journal/index.php/rib/article/view/2423 SP - AB - <em>In vitro</em> regeneration of medicinally important plant <em>Eryngium foetidum</em> L. was established by developing a protocol for successful faster clonal propogation. Young leaves of the plant were taken and cultured on 1x MS medium supplemented with different concentrations and combinations of 6- Benzyl amino purine (BAP), 1-Napthylacetic acid (NAA) and Kinetin (Kin). Leaves cultured on 2mg/L BAP and 1mg/L NAA showed better response in short duration of time when compared to other concentrations and combinations. Induced shoots obtained from standardized media were used as explant and cultured on 1x MS medium containing 2mg/L BAP and 1mg/L NAA fortified with 10% of different nitrogen sources like  tryptone, peptone, beef extract, yeast extract and adjuvants like coconut milk, tomato juice and banana pulp to observe the growth response. It was observed that media containing 10% coconut milk and 10% peptone showed better result when compared to other adjuvants and nitrogen sources. The well developed plantlets were hardened and successfully transferred to the field with 85% survival rate. ER -