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TY  - JOUR
AU  - A, Aparna
AU  - Srinikethan, G.
AU  - Hegde, Smitha
PY  - 2012/03/02
Y2  - 2025/09/23
TI  - Isolation, Screening and Production of Biosurfactant by Bacillus clausii 5B
JF  - Research in Biotechnology
JA  - RIB
VL  - 3
IS  - 2
SE  - Research Articles
DO  - 
UR  - https://www.updatepublishing.com/journal/index.php/rib/article/view/2397
SP  - 
AB  - Primary somatic embryos induced from the callus tissues revealed the presence of CSSV between 12-24 wks by PCR/capillary electrophoresis while PCR/agarose electrophoresis failed to detect the virus. Different carbon and nitrogen sources were evaluated for their effect on biosurfactant production. Maximum biosurfactant synthesis (2.11 g/L) was observed at 96 hours when the cells were grown on minimal medium containing 1 % (w/v) glucose as carbon source. Among nitrogen sources tested, ammonium chloride showed maximum biosurfactant production of 2.41 g/L. The biosurfactant produced by the bacterial isolate reduced the surface tension of the cell free broth from 53.56 mN/m to 29.48 mN/m. Compositional analysis of the biosurfactant revealed that it was of lipopetide type, composed of high percentage of lipid (~56 %, w/w) and protein (~39 %, w/w) content.
ER  -