Warning: ini_set(): A session is active. You cannot change the session module's ini settings at this time in /home/updatepublishing/public_html/journal/lib/pkp/classes/session/SessionManager.inc.php on line 69
Warning: Cannot modify header information - headers already sent by (output started at /home/updatepublishing/public_html/journal/lib/pkp/classes/session/SessionManager.inc.php:69) in /home/updatepublishing/public_html/journal/plugins/generic/citationStyleLanguage/CitationStyleLanguagePlugin.inc.php on line 478
Warning: Cannot modify header information - headers already sent by (output started at /home/updatepublishing/public_html/journal/lib/pkp/classes/session/SessionManager.inc.php:69) in /home/updatepublishing/public_html/journal/plugins/generic/citationStyleLanguage/CitationStyleLanguagePlugin.inc.php on line 479
TY - JOUR
AU - Kumar, Kapila
AU - Rana, Jyoti
AU - Guleria, Ayushi
AU - Gupta, Amita
AU - Chaudhary, Vijay K.
AU - Gupta, Sanjay
PY - 2011/12/24
Y2 - 2025/09/23
TI - Expression and Characterization of Chandipura Virus Proteins
JF - Research in Biotechnology
JA - RIB
VL - 2
IS - 6
SE - Research Articles
DO -
UR - https://www.updatepublishing.com/journal/index.php/rib/article/view/2375
SP -
AB - Chandipura virus (CHPV) has recently emerged as an extremely lethal human pathogen in the family <em>Rhabdoviridae </em>and is linked to significant encephalitis outbreaks in different parts of India. The biology of CHPV remains less studied to date and the availability of reagents such as purified proteins can enhance research in this direction. In this study, we have overexpressed four of the CHPV proteins namely Nucleoprotein (N), Phosphoprotein (P), Matrix protein (M) and Glycoprotein (G) using three distinct tags in bacterial system and with changes in inducer concentration, growth and solubilisation conditions successfully purified M and G proteins for the first time along with N and P. Furthermore, the interactions of CHPV M protein with other viral proteins (G, N and P) was investigated using ELISA and GST pull down assays to show the utility of olubilised proteins. The results of both the assays demonstrated that M protein interacts with both G and N proteins, while it does not interact with P protein, in a similar manner as reported for Vesicular Stomatitis Virus.
ER -