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TY - JOUR AU - Kumar, Owk Aniel AU - Jyothirmayee, Geddam AU - Tata, Sape Subba PY - 2011/10/24 Y2 - 2025/09/23 TI - In vitro plant regeneration from leaf explants of Withania somnifera (L) Dunal (Ashwaganda) - an important medicinal plant JF - Research in Biotechnology JA - RIB VL - 2 IS - 5 SE - Research Articles DO - UR - https://www.updatepublishing.com/journal/index.php/rib/article/view/2369 SP - AB - <em>Withania somnifera</em> (L) Dunal (Ashwaganda) is a Solanaceous herb having numerous medicinal values widely used in traditional Ayurvedic drug preparations. An efficient protocol for <em>in vitro</em> plant regeneration via direct adventitious shoot proliferation from leaf explants of Ashwaganda is developed. MS medium containing 1.5mg/l BAP and 1.5mg/l IAA was found to be the best medium for maximum <em>in vitro</em> response i.e., 100(% shooting) and 68(shoots/explant). An improved <em>in vitro</em> shoot bud elongation and rooting was achieved on MS medium fortified with 0.15mg/l GA3 and 5mg/l IBA  respectively. Rooted plants were hardened and transplanted in earthen pots and were showed 80-90% survival during transplantation. ER -