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TY - JOUR AU - Quainoo, A. K. PY - 2011/04/15 Y2 - 2025/09/23 TI - Age of callus tissues and cotyledonary materials on the selection of cocoa swollen shoot virus-free somatic embryos JF - Research in Biotechnology JA - RIB VL - 2 IS - 2 SE - Research Articles DO - UR - https://www.updatepublishing.com/journal/index.php/rib/article/view/2339 SP - AB - Research was conducted to investigate the effect of age of callus tissue and cotyledonary material on the selection of CSSV-free cocoa somatic embryos. Polymerase chain reaction (PCR) capillary electrophoresis was more sensitive and quick in detecting the CSSV than PCR/agarose electrophoresis. PCR/capillary electrophoresis revealed the presence of CSSV in callus tissues in 1wk at the rate of 53(82 %), 47(94 %) and 46(85 %) for three infected Amelonado cocoa trees, T1, T2 and T4, respectively while  PCR/agarose electrophoresis recorded 23(36 %), 19(38 %) and 26(48 %) for T1, T2 and T4), respectively. ER -