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@article{Rudramurthy_Sengupta_Lakkundi_Ligi_D’Souza_Rahman_2014, title={Sequencing and analysis of invariant surface glycoprotein (ISG) gene from Trypanosoma evansi dog isolate}, volume={6}, url={https://www.updatepublishing.com/journal/index.php/rib/article/view/2654}, abstractNote={<p><em>Trypanosoma (T) evansi</em> causes a chronic wasting disease called “surra” in cattle, buffaloes, horses, canines, felines etc. In the present study <em>T.evansi</em> isolated from dog from Karnataka state in India was used to sequence invariant surface glycoprotein (ISG) gene and the obtained sequence was analyzed to elucidate its relationship with other isolates/ species. The  ISG gene sequences obtained from four recombinant clones revealed the open reading frame (ORF) of  1521  nucleotide (nt) encoding a polypeptide of 506 amino acids (aa) and belongs ISG-75 gene family. Sequence analysis revealed 92-99% and 65-99% similarity at nt and aa levels, respectively with other isolates / species and belongs to the RoTat 1.2 strain</p>}, number={1}, journal={Research in Biotechnology}, author={Rudramurthy, G R and Sengupta, Pinaki P and Lakkundi, J N and Ligi, M and D’Souza, P E and Rahman, H}, year={2014}, month={Dec.} }