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@article{Ravinder_Prabhakar_Prashanthkumar_Venuka_2014, title={Optimization of process parameters of cyclodextrin glycosyltransferase isolated from novel mutated Bacillus sp. TPR71HNA6 by Taguchi orthogonal array method}, volume={5}, url={https://www.updatepublishing.com/journal/index.php/rib/article/view/2455}, abstractNote={<p><strong>Cyclodextrin glycosyl transferase CGTase) production by B</strong><strong><em>acillus</em></strong><strong> SP. TPR71HNA6 </strong><strong>under shake flask culture was optimized following Taguchi orthogonal array (OA) design of experiment.  An OA layout of L16 was constructed with five most influensive factors on CGTase like </strong><strong>pH, initial temperature, agitation speed, time of incubation and inoculum concentration</strong><strong> at three levels for the proposed experimental design. </strong><strong>According to the experimental plan (L-16) the experiments were conducted and the data revealed significant variation in the enzyme production. Minimum and maximum CGTase production values were observed to be 19 and 89 U/mL under the above fermentation conditions. The observed variation certainly indicates the imperative role of optimization of all fermentation-related factors in achieving the best possible yields. </strong><strong>With the help of statistical tool by optimizing the process variables without altering the nutritional compounds and their concentration an overall 39% of enzyme yield was improved. </strong></p>}, number={3}, journal={Research in Biotechnology}, author={Ravinder, Kashipeta and Prabhakar, Tadimalla and Prashanthkumar, Katikala and Venuka, Nalli}, year={2014}, month={Jun.} }