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@article{Ibrahim_Mukhtar_Ibrahim_Oke_Ajijolakewu_2012, title={<em>Adansonia digitata</em> (Baobab) fruit pulp as substrate for <em>Bacillus</em> Endoglucanase production}, volume={3}, url={https://www.updatepublishing.com/journal/index.php/rib/article/view/2392}, abstractNote={In recent years the cost of carbon source for microbial enzymes production has necessitated a drive towards cheaper and sustainable sources. In this study, &lt;em&gt;Adansonia digitata&lt;/em&gt; (Baobab) fruit pulp was utilized as substrate for &lt;em&gt;Bacillus &lt;/em&gt;endoglucanase production and the effects of varying temperature, pH, incubation period, inoculum size and substrate concentration on endoglucanase production were investigated. The cellulolytic activity of the isolates was screened base on halo of clearance on Carboxymethyl  cellulose agar, while the endoglucanase activity of the selected &lt;em&gt;Bacillus&lt;/em&gt; species was monitored using 3, 5 - dinitrosalicylic acid (DNS) method. Out of 4 &lt;em&gt;Bacillus &lt;/em&gt;species screened, only &lt;em&gt;Bacillus amyloliquefaciens&lt;/em&gt; and &lt;em&gt;Bacillus subtitis&lt;/em&gt; showed cellulolytic activity with &lt;em&gt;B. amyloliquefaciens&lt;/em&gt; having the highest activity of 20.0 mm. Therefore&lt;em&gt; B. amyloliquefaciens&lt;/em&gt; was selected  for studies on the effect of fermentation conditions on endoglucanase activity. The maximum yield of endoglucanase was produced at temperature of 55 oC, pH 2, 1% inoculum size and 1% substrate concentration for 4 days incubation period. The results of this study  suggest that &lt;em&gt;Adansonia digitata&lt;/em&gt; fruit pulp can be harnessed at low concentration for large scale endoglucanase production by &lt;em&gt;Bacillus&lt;/em&gt;, and the endoglucanase produced by &lt;em&gt;B. amyloliquefaciens&lt;/em&gt; could be a thermostable enzyme with novel characteristics suitable for application in biofuel and textile industry.}, number={2}, journal={Research in Biotechnology}, author={Ibrahim, A.D. and Mukhtar, Sa’adat I. and Ibrahim, M.N. and Oke, M. A. and Ajijolakewu, A.K}, year={2012}, month={Mar.} }