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@article{Kumar_Jyothirmayee_Tata_2011, title={<em>In vitro</em> plant regeneration from leaf explants of <em>Withania somnifera</em> (L) Dunal (Ashwaganda) - an important medicinal plant}, volume={2}, url={https://www.updatepublishing.com/journal/index.php/rib/article/view/2369}, abstractNote={<em>Withania somnifera</em> (L) Dunal (Ashwaganda) is a Solanaceous herb having numerous medicinal values widely used in traditional Ayurvedic drug preparations. An efficient protocol for <em>in vitro</em> plant regeneration via direct adventitious shoot proliferation from leaf explants of Ashwaganda is developed. MS medium containing 1.5mg/l BAP and 1.5mg/l IAA was found to be the best medium for maximum <em>in vitro</em> response i.e., 100(% shooting) and 68(shoots/explant). An improved <em>in vitro</em> shoot bud elongation and rooting was achieved on MS medium fortified with 0.15mg/l GA3 and 5mg/l IBA respectively. Rooted plants were hardened and transplanted in earthen pots and were showed 80-90% survival during transplantation.}, number={5}, journal={Research in Biotechnology}, author={Kumar, Owk Aniel and Jyothirmayee, Geddam and Tata, Sape Subba}, year={2011}, month={Oct.} }