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TY - JOUR AU - C.V. Naidu*, T.M. Sridhar and PY - 2011/03/19 Y2 - 2025/09/23 TI - Effect of Different Carbon Sources on In vitro Shoot Regeneration of Solanum nigrum (Linn) - An Important Antiulcer Medicinal Plant JF - Journal of Phytology JA - jp VL - 3 IS - 2 SE - Tissue Culture DO - UR - https://www.updatepublishing.com/journal/index.php/jp/article/view/2242 SP - AB - <p class="MsoNormal" style="margin: 0in 0.5in 0pt; text-align: justify;"><span style="font-size: 9.5pt; font-family: ";Book Antiqua";,";serif";;">An efficient <em>in vitro</em> protocol for mass propagation of <em>Solanum nigrum</em> was developed.<span style="mso-spacerun: yes;">  </span>In the present study, the effect of various carbon sources, sucrose, glucose, fructose, and maltose was investigated on <em>in vitro </em>shoot regeneration using nodal explants. The frequency, growth and multiplication rate were highly influenced by the type and concentration of carbon sources used.<span style="mso-spacerun: yes;">  </span>The highest number of shoots (24.0</span><span style="font-size: 9.5pt; font-family: Symbol; mso-ascii-font-family: 'Book Antiqua'; mso-hansi-font-family: 'Book Antiqua'; mso-char-type: symbol; mso-symbol-font-family: Symbol;"><span style="mso-char-type: symbol; mso-symbol-font-family: Symbol;">±</span></span><span style="font-size: 9.5pt; font-family: ";Book Antiqua";,";serif";;">0.28) were obtained on MS medium supplemented with 4% fructose, but maximum shoot length (11.0</span><span style="font-size: 9.5pt; font-family: Symbol; mso-ascii-font-family: 'Book Antiqua'; mso-hansi-font-family: 'Book Antiqua'; mso-char-type: symbol; mso-symbol-font-family: Symbol;"><span style="mso-char-type: symbol; mso-symbol-font-family: Symbol;">±</span></span><span style="font-size: 9.5pt; font-family: ";Book Antiqua";,";serif";;">0.28 cm) was observed on MS medium supplemented with 4% sucrose. The least number of shoots obtained on MS medium supplemented with 1% glucose (3.5</span><span style="font-size: 9.5pt; font-family: Symbol; mso-ascii-font-family: 'Book Antiqua'; mso-hansi-font-family: 'Book Antiqua'; mso-char-type: symbol; mso-symbol-font-family: Symbol;"><span style="mso-char-type: symbol; mso-symbol-font-family: Symbol;">±</span></span><span style="font-size: 9.5pt; font-family: ";Book Antiqua";,";serif";;">0.50), with a shoot length of (3.4</span><span style="font-size: 9.5pt; font-family: Symbol; mso-ascii-font-family: 'Book Antiqua'; mso-hansi-font-family: 'Book Antiqua'; mso-char-type: symbol; mso-symbol-font-family: Symbol;"><span style="mso-char-type: symbol; mso-symbol-font-family: Symbol;">±</span></span><span style="font-size: 9.5pt; font-family: ";Book Antiqua";,";serif";;">0.34 cm). Among the different carbon sources used in the present study, fructose at 4% proved to be better choice for multiple shoot regeneration followed by sucrose, maltose and glucose, from nodal explants of <em>Solanum nigrum</em>.</span></p> ER -