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TY - JOUR AU - Balasubramanya S3 and Anuradha M1,2, Sudipta K.M1, Kumara Swamy M2*, PY - 2011/03/05 Y2 - 2025/09/23 TI - Cost Effective Approach for In vitro Propagation of (Leptadenia reticulata Wight &Arn.) - A Threatened Plant of Medicinal Importance JF - Journal of Phytology JA - jp VL - 3 IS - 2 SE - Tissue Culture DO - UR - https://www.updatepublishing.com/journal/index.php/jp/article/view/2241 SP - AB - <p class="MsoNormal" style="margin: 6pt 31.25pt 0pt 32.2pt; text-indent: 0in; line-height: normal;"><span style="font-size: 10pt; font-family: ">The present study deals with the development of an efficient and simple protocol for high frequency <em style="mso-bidi-font-style: normal;">in vitro</em> regeneration of <em style="mso-bidi-font-style: normal;">Leptadenia reticulata</em>, a threatened medicinal plant. A range of cytokinins with different concentration and affect of various media have been investigated for multiple shoot induction using nodes, internodes, meristem as explants. The best response for multiplication was obtained in MS media supplemented with 0.25mg/l BA and 0.25mg/l Kn. B5 media efficiently proliferated callus. Among the all the explants tested only those of old axillary nodes showed positive morphogenetic response and readily developed healthy multiple shoots, where as the other explants such as young axillary nodes, apical meristem and internodes did not respond satisfactorily. Maximum numbers of roots were observed when the <em style="mso-bidi-font-style: normal;">in vitro</em> grown shoots were maintained on full strength MS media containing 2mg/l IBA followed by 200 mg/l activated charcoal. The cost of the media was reduced by using tap water and table sugar in the media. About 93% of the plants were successfully acclimatized in the field. </span></p><p class="MsoNormal" style="margin: 6pt 0in 0pt; text-indent: 0in; line-height: normal;"><strong><span style="font-size: 10pt; font-family: ">Key words: </span></strong><span style="font-size: 10pt; font-family: ">Multiple shoot induction, Axillary nodes, <em style="mso-bidi-font-style: normal;">In vitro</em> regeneration, Asclepiadaceae<em style="mso-bidi-font-style: normal;"> </em></span></p><p class="MsoNormal" style="margin: 6pt 0in 0pt; text-indent: 0in; line-height: normal;"><strong><span style="font-size: 10pt; font-family: ">Abbreviations: </span></strong><span style="font-size: 10pt; font-family: ">BA: 6-benzyladenine, IAA: Indole-3-aceticacid, IBA: Indole-3-butyric acid, Kn: Kinetin, NAA: α-naphthalene acetic acid, MS: Murashige&Skoog’s medium, SE.: Standard error</span></p><h1 style="text-justify: inter-ideograph; margin: 6pt 0in 0pt; text-align: justify;"><span style="font-weight: normal; font-size: 7pt; line-height: 110%; font-family: " lang="EN-SG">Sudipta K.M et al. Cost Effective Approach for In vitro Propagation of (<em style="mso-bidi-font-style: normal;">Leptadenia reticulata</em> Wight &Arn.) - A Threatened Plant of Medicinal Importance. J Phytol 3/2 (2011) 72-77</span></h1> ER -