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TY - JOUR AU - Kota, V.R. Kokkirala, K. Kasula, S. Abbagani*, K. Gadidasu, P. Umate, M. Aileni, S.R. PY - 2011/02/28 Y2 - 2025/09/23 TI - Micropropagation of a Valuable Ethnomedicinal Plant Streblus asper Lour. JF - Journal of Phytology JA - jp VL - 3 IS - 2 SE - Tissue Culture DO - UR - https://www.updatepublishing.com/journal/index.php/jp/article/view/2240 SP - AB - <p class="MsoNormal" style="margin: 6pt 31.25pt 0pt 32.2pt; text-indent: 0in; line-height: normal;"><span style="font-size: 10pt; font-family: ";Book Antiqua";,";serif";;">A micropropagation protocol is presented for conservation of critically threatened woody tree species, <em>Streblus asper </em>Lour. <em>In vitro</em> axillary bud proliferation followed by multiple shoot induction was obtained using mature nodal segments. Initially, explants were cultured on Murashige and Skoog’s medium supplemented with different concentrations of BA (2.2, 4.4, 6.6, 8.9, 11.1 and 13.3 µM), Kn (2.3, 4.6, 6.9, 9.3, 11.6 and 13.9 µM) or TDZ (2.2, 4.5, 6.7, 8.90, 11.1 and 13.5 µM). These individual levels of cytokinins did not support <em>in vitro </em>shoot regeneration in <em>S. asper</em>. Combinations of cytokinins, Kn with BA or TDZ, significantly influenced shoot regeneration ability. The combination of Kn (4.60 µM) with BA (4.44 µM) evoked an optimum response towards shoot proliferation whereas, medium containing Kn (4.60 µM) plus TDZ (4.54 µM) induced multiple shoot formation. <em>In vitro</em> developed microshoots were rooted on MS half strength medium supplemented with 2.46 µM IBA. The plantlets established <em>in vitro</em> were transferred to pots containing sterilized soil and vermiculite (1:1) mixture and were hardened in the greenhouse with 70-75% survival rate. </span></p><p class="MsoNormal" style="margin: 6pt 0in 0pt; text-indent: 0in; line-height: normal;"><strong><span style="font-size: 10pt; font-family: ";Book Antiqua";,";serif";;">Key words: </span></strong><span style="font-size: 10pt; font-family: ";Book Antiqua";,";serif";;">Clonal propagation, Cytokinins, Mature nodal explant, Medicinal woody tree, Multiple shoot induction </span></p><p class="MsoNormal" style="margin: 6pt 0in 0pt; text-indent: 0in; line-height: normal;"><strong><span style="font-size: 10pt; font-family: ";Book Antiqua";,";serif";;">Abbreviations: </span></strong><span style="font-size: 10pt; font-family: ";Book Antiqua";,";serif";;">BA - 6-benzyladenine, IBA - indole 3-butyric acid, Kn - kinetin, µM - micro moles, MS – Murashige and Skoog TDZ - thidiazuron<strong> </strong></span></p><h1 style="text-justify: inter-ideograph; margin: 6pt 0in 0pt; text-align: justify;"><span style="font-weight: normal; font-size: 7pt; line-height: 110%; font-family: ";Book Antiqua";,";serif";; letter-spacing: -0.3pt; font-variant: normal! important; mso-fareast-language: EN-SG; mso-font-kerning: 0pt; mso-no-proof: no; mso-ansi-language: EN-SG;" lang="EN-SG">Kranthi Gadidasu et al. Micropropagation of a Valuable Ethnomedicinal Plant Streblus asper Lour.. J Phytol 3/2 (2011) 18-23</span></h1> ER -