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TY  - JOUR
AU  - Saini, Gulshan Saini and Amla Batra, Rajendra Saini*, Anirudha Rishi, Shikha
PY  - 2010/11/27
Y2  - 2025/09/23
TI  - Rapid in vitro Propagation of Boerhaavia diffusa (L.) through Nodal Segments
JF  - Journal of Experimental Sciences
JA  - JES
VL  - 1
IS  - 8
SE  - Life Sciences
DO  - 
UR  - https://www.updatepublishing.com/journal/index.php/jes/article/view/1754
SP  - 
AB  - &lt;div class=&quot;Section1&quot;&gt;&lt;p class=&quot;MsoNormal&quot; style=&quot;text-align: justify; margin: 0in 0in 0pt; mso-layout-grid-align: none;&quot;&gt;Â &lt;/p&gt;&lt;span style=&quot;font-family: &quot;;Tahoma&quot;;,&quot;;sans-serif&quot;;; font-size: 6pt;&quot;&gt;&lt;div style=&quot;border-bottom: medium none; border-left: medium none; padding-bottom: 0in; padding-left: 0in; padding-right: 0in; background: white; border-top: windowtext 1pt solid; border-right: medium none; padding-top: 1pt; mso-border-top-alt: solid windowtext .5pt; mso-element: para-border-div;&quot;&gt;&lt;p class=&quot;MsoNormal&quot; style=&quot;text-align: justify; margin: 6pt 0in 0pt; background: white; mso-border-top-alt: solid windowtext .5pt; mso-padding-alt: 1.0pt 0in 0in 0in; padding: 0in;&quot;&gt;&lt;span style=&quot;font-family: &quot;;Tahoma&quot;;,&quot;;sans-serif&quot;;; font-size: 8pt;&quot;&gt;A rapid and efficient protocol for the large scale propagation of a potential medicinal plant &lt;em style=&quot;mso-bidi-font-style: normal;&quot;&gt;Boerhaavia diffusa&lt;/em&gt; L., through &lt;em style=&quot;mso-bidi-font-style: normal;&quot;&gt;in vitro&lt;/em&gt; culture of nodal segment explants obtained from aseptic seedlings. &lt;em style=&quot;mso-bidi-font-style: normal;&quot;&gt;In vitro&lt;/em&gt; multiple shoot (14-15) induction was observed from axillary bud explants cultured on MS medium fortified with BAP (2.0 mg/l) and Kn (3.5 mg/l). The multiple shoots were separated and subcultured for their elongation on same medium supplemented with gibberelic acid (0.5 mg/l). Rooting on &lt;em style=&quot;mso-bidi-font-style: normal;&quot;&gt;in vitro&lt;/em&gt; produced elongated shoots was achieved on half MS medium having IBA (0.5 mg/l). Rooted plantlets were hardened in plastic pots containing sterilized soil and vermiculite (3:1). Well established plantlets were acclimatized to the field with 70% survival rate. &lt;/span&gt;&lt;/p&gt;&lt;/div&gt;&lt;p class=&quot;MsoNormal&quot; style=&quot;text-align: justify; margin: 0in 0in 0pt;&quot;&gt;&lt;span style=&quot;font-family: &quot;;Tahoma&quot;;,&quot;;sans-serif&quot;;; font-size: 8pt;&quot;&gt;Â &lt;/span&gt;&lt;/p&gt;&lt;p class=&quot;MsoNormal&quot; style=&quot;margin: 0in 0in 0pt;&quot;&gt;&lt;strong style=&quot;mso-bidi-font-weight: normal;&quot;&gt;&lt;span style=&quot;font-family: &quot;;Tahoma&quot;;,&quot;;sans-serif&quot;;; font-size: 8pt;&quot;&gt;Key words:&lt;/span&gt;&lt;/strong&gt;&lt;em style=&quot;mso-bidi-font-style: normal;&quot;&gt;&lt;span style=&quot;font-family: &quot;;Tahoma&quot;;,&quot;;sans-serif&quot;;; font-size: 8pt;&quot;&gt; &lt;/span&gt;&lt;/em&gt;&lt;span style=&quot;font-family: &quot;;Tahoma&quot;;,&quot;;sans-serif&quot;;; font-size: 8pt;&quot;&gt;Medicinal plants, Nodal segments, Micropropagation, &lt;em&gt;in vitro&lt;/em&gt; rooting &lt;/span&gt;&lt;/p&gt;&lt;p class=&quot;MsoNormal&quot; style=&quot;text-align: justify; margin: 0in 0in 0pt; mso-layout-grid-align: none;&quot;&gt;Â &lt;/p&gt;&lt;p class=&quot;MsoNormal&quot; style=&quot;text-align: justify; margin: 0in 0in 0pt; mso-layout-grid-align: none;&quot;&gt;Plant biotechnology laboratory, Department of Botany, University of Rajasthan, Jaipur 302004, Rajasthan&lt;/p&gt;&lt;/span&gt;&lt;/div&gt;&lt;strong style=&quot;mso-bidi-font-weight: normal;&quot;&gt;&lt;span style=&quot;font-family: &quot;;Tahoma&quot;;,&quot;;sans-serif&quot;;; font-size: 8pt; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;&quot;&gt;&lt;br style=&quot;page-break-before: always; mso-break-type: section-break;&quot; /&gt;&lt;/span&gt;&lt;/strong&gt;
ER  -