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@article{Arenmongla_2011, title={Morphogenetic induction and organogenic differentiation from foliar explants of Strobilanthes flaccidifolious Nees: a natural dye yielding plant}, volume={2}, url={https://www.updatepublishing.com/journal/index.php/jes/article/view/1888}, abstractNote={<p class="MsoNormal" style="text-indent: 0in; margin: 0in 34.3pt 0pt 35.45pt; mso-char-indent-count: 0;"><span style="font-size: x-small;"><em style="mso-bidi-font-style: normal;"><span style="font-family: ";Arial Narrow";,";sans-serif";;">Strobilanthes flaccidifolious</span></em><span style="font-family: ";Arial Narrow";,";sans-serif";;"> is a shrub and its leaf is the source of blue color natural dye used for dying fibers and play important role in handloom industry. During the present investigation, an efficient <em style="mso-bidi-font-style: normal;">in vitro</em> regeneration protocol is developed from foliar segments of <em style="mso-bidi-font-style: normal;">in vitro</em> sourced plant materials. The first sign of response from the cultured foliar segments was recorded as swelling and curling of leaf segments within 7 days of culture followed by callusing and shoot buds formation within 3-4 wk. Amongst the different plant growth regulators tested, optimum response was recorded on MS medium enriched with 3% sucrose, 6 µM BA while other growth regulators and their concentrations did not support satisfactory morphogenetic response. Under optimum condition ~75% explants responded positively where as many as 9 shoots buds developed per leaf segment. The shoot buds converted into plantlets/micro shoots on MS medium when supplemented with 3 µM Kn where as many as 7.3 shoot buds developed per subculture of 3-4 wk duration. The micro shoots induced roots on MS medium enriched with 3 µM NAA where as many as 14 roots developed per plant within 4 wk times. The well rooted plantlets were transferred in potting mix and maintained in the poly-shed (50% light) for two months before transferring to the field.<span style="mso-spacerun: yes;">    </span><em style="mso-bidi-font-style: normal;"><span style="mso-spacerun: yes;"> </span></em></span></span></p>}, number={10}, journal={Journal of Experimental Sciences}, author={Arenmongla, Chitta Ranjan Deb* and T.}, year={2011}, month={Oct.} }