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@article{T. Sellathurai*_2010, title={In vitro Regeneration and Phytochemical Screening of Tylophora indica, an Endangered Medicinal Herb}, volume={1}, url={https://www.updatepublishing.com/journal/index.php/jes/article/view/1766}, abstractNote={<div style="border-bottom: medium none; border-left: medium none; padding-bottom: 0in; padding-left: 0in; padding-right: 0in; background: white; border-top: windowtext 1pt solid; border-right: medium none; padding-top: 1pt; mso-element: para-border-div; mso-border-top-alt: solid windowtext .5pt;"><p class="MsoNormal" style="text-align: justify; margin: 6pt 0in 0pt; background: white; mso-border-top-alt: solid windowtext .5pt; mso-padding-alt: 1.0pt 0in 0in 0in; padding: 0in;"><strong style="mso-bidi-font-weight: normal;"><span style="font-family: ";Tahoma";,";sans-serif";; font-size: 8pt;">ABSTRACT</span></strong><span style="font-family: ";Tahoma";,";sans-serif";; font-size: 8pt;">: <em>In vitro</em> regeneration of <em>Tylophora indica</em> from mature leaf explants placed on MS with low concentration (1</span><span style="font-family: Symbol; font-size: 8pt; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma; mso-bidi-font-family: Tahoma; mso-char-type: symbol; mso-symbol-font-family: Symbol;"><span style="mso-char-type: symbol; mso-symbol-font-family: Symbol;">m</span></span><span style="font-family: ";Tahoma";,";sans-serif";; font-size: 8pt;">M) of Kin and BA alone and in combination with Kin and BA induced multiple shoots and roots. Leaf explants on MS with BA<span style="mso-spacerun: yes;">Â Â </span>and 2, 4-D or NAA along with Ads had poor organogenic responses. The responses of leaf explants in MS + Kin (1</span><span style="font-family: Symbol; font-size: 8pt; mso-ascii-font-family: Tahoma; mso-hansi-font-family: Tahoma; mso-bidi-font-family: Tahoma; mso-char-type: symbol; mso-symbol-font-family: Symbol;"><span style="mso-char-type: symbol; mso-symbol-font-family: Symbol;">m</span></span><span style="font-family: ";Tahoma";,";sans-serif";; font-size: 8pt;">M) implicate the feasibility for multiplication of this species. Comparative phytochemical analysis revealed the presence of alkaloids, steroids and carbohydrates both in adult leaf and <em style="mso-bidi-font-style: normal;">in vitro </em>derived callus. It evidences the potential utility of tissue cultures for production of bioactive compounds. It may be concluded that further standardization of hormonal combinations could<span style="mso-spacerun: yes;">Â Â </span>helpful for large scale propagation and extraction of drugs for pharmaceutical application.</span><span style="mso-spacerun: yes;"><span style="font-family: Times New Roman; font-size: small;">Â Â </span></span><span style="font-family: ";Tahoma";,";sans-serif";; font-size: 8pt;"></span></p></div>}, number={11}, journal={Journal of Experimental Sciences}, author={T. Sellathurai*, S. Rathinavel and}, year={2010}, month={Dec.} }