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@article{Ajit Kumar_2010, title={In vivo and In vitro Estimation of Colchicine in Gloriosa superba L. by High Pressure Liquid Chromatography}, volume={1}, url={https://www.updatepublishing.com/journal/index.php/jes/article/view/1709}, abstractNote={<div style="border-right: medium none; padding-right: 0in; border-top: windowtext 1pt solid; padding-left: 0in; background: white; padding-bottom: 0in; border-left: medium none; padding-top: 1pt; border-bottom: medium none; mso-element: para-border-div; mso-border-top-alt: solid windowtext .5pt;"><p class="MsoNormal" style="background: white; margin: 6pt 0in 0pt; text-align: justify; mso-border-top-alt: solid windowtext .5pt; mso-padding-alt: 1.0pt 0in 0in 0in; padding: 0in;"><span style="font-size: 8pt; color: black; font-family: ";Tahoma";,";sans-serif";; letter-spacing: -0.2pt;">The presence of Colchicine in <em>Gloriosa superba</em> was confirmed by Thin Layer Chromatography (TLC) and High Pressure Liquid Chromatography (HPLC) in which a spot coinciding with the authentic sample of Cochicine in Rf value (Colchicine 0.70) appeared. The maximum amount of Colchicine was found in flower (20.7 mg/gdw) and minimum amount was found in stem (7.4 mg/gdw).The In vitro studies showed the maximum amount of Colchicine in 6 week old cultures (22.6 mg/gdw)) and minimum amount in 2 weeks old cultures (15.3 mg/gdw). </span></p></div><p class="MsoNormal" style="margin: 6pt 0in 0pt;"><strong style="mso-bidi-font-weight: normal;"><span style="font-size: 8pt; font-family: ";Tahoma";,";sans-serif";;">Key words:</span></strong><em style="mso-bidi-font-style: normal;"><span style="font-size: 8pt; font-family: ";Tahoma";,";sans-serif";;"> </span></em><span style="font-size: 8pt; font-family: ";Tahoma";,";sans-serif";;">Colchicine, <em style="mso-bidi-font-style: normal;">Gloriosa superba</em>, HPLC </span></p><div style="border-right: medium none; padding-right: 0in; border-top: windowtext 1pt solid; padding-left: 0in; padding-bottom: 0in; border-left: medium none; padding-top: 1pt; border-bottom: medium none; mso-element: para-border-div; mso-border-top-alt: solid windowtext .5pt;"><p class="MsoNormal" style="margin: 6pt 0in 0pt; text-align: justify; mso-layout-grid-align: none;"><span style="font-size: 6pt; font-family: ’Tahoma’,’sans-serif’;">Laboratory of Bioactive Natural Products, Department of Botany, University of Rajasthan, Jaipur -302004 </span></p><p class="MsoNormal" style="margin: 6pt 0in 0pt; text-align: justify; mso-layout-grid-align: none;"><span style="font-size: 6pt; font-family: ’Tahoma’,’sans-serif’;"><span style="font-size: 8pt; font-family: ’Tahoma’,’sans-serif’; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;"><em>* Corresponding Author, Email: renusarin@sify.com; Tel: +91-141-2701875, +91-9828070584 </em></span></span></p><span style="font-size: 10pt; font-family: ";Tahoma";,";sans-serif";;"><p class="MsoNormal" style="margin: 6pt 0in 0pt 0.25in; text-indent: -18.75pt; text-align: justify;"><span style="text-decoration: underline;"><span style="font-size: 8pt; color: black; font-family: ";Tahoma";,";sans-serif";; mso-bidi-font-weight: bold;">Please Cite This Article As:</span></span></p><p class="MsoNormal" style="margin: 6pt 0in 0pt -0.9pt; text-indent: 0.15pt; text-align: justify;"><span style="font-size: 8pt; color: black; font-family: ";Tahoma";,";sans-serif";; mso-bidi-font-weight: bold;">Renu Sarin, Anirudha Rishi and Ajit Kumar. 2010. In vivo and In vitro Estimation of Colchicine in <em>Gloriosa superba</em> L. by High Pressure Liquid Chromatography. J. Exp. Sci. 1(4):1-2.</span></p><p class="MsoNormal" style="margin: 6pt 0in 0pt; text-align: justify; mso-layout-grid-align: none; mso-border-top-alt: solid windowtext .5pt; mso-padding-alt: 1.0pt 0in 0in 0in; padding: 0in;">Â </p></span></div>}, number={4}, journal={Journal of Experimental Sciences}, author={Ajit Kumar, Renu Sarin*, Anirudha Rishi and}, year={2010}, month={Sep.} }