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TY - JOUR AU - and C.V.Naidu3*, D. Preethi1, T.M.Sridhar2, P.Josthna2 PY - 2011/07/04 Y2 - 2025/09/23 TI - Studies on Antibacterial Activity, Phytochemical Analysis of Stevia rebaudiana (Bert.) - An Important Calorie Free Biosweetner JF - Journal of Ecobiotechnology JA - JEBT VL - 3 IS - 7 SE - Microbial Biotechnology DO - UR - https://www.updatepublishing.com/journal/index.php/jebt/article/view/183 SP - AB - <p class="MsoNormal" style="margin: 6pt 0in 0pt; text-align: justify;"><em><span style="font-size: 10pt; font-family: ";Arial Narrow";,";sans-serif";;">Stevia rebaudiana </span></em><span style="font-size: 10pt; font-family: ";Arial Narrow";,";sans-serif";;">is an outstanding herb bearing leaves of very refreshing sweet taste and remarkable health promoting activities.<span style="mso-spacerun: yes;">  </span>The herb is nutrient rich, containing substantial amounts of protein, calcium, phosphorous, sodium, magnesium, zinc, rutin, vitamin A, vitamin C and other nutrients, yet has no caloric value.<span style="mso-spacerun: yes;">  </span>Six solvent extracts from leaf, three solvent extracts from flower of stevia were assayed for <em>in vitro</em> antibacterial activity against pathogenic bacteria such as <em>Bacillus subtilis, Klebsiella pneumonia, Proteus vulgaris, Streptococcus pneumoniae, Staphylococcus aureus </em>and <em>Pseudomonas fluorescence, </em>the zone of inhibition were compared with different standard antibiotics.<span style="mso-spacerun: yes;">  </span>Phytochemical screening of the crude extracts revealed the presence of secondary compounds such as alkaloids, flavonids, steroids, tannins and phenols. The organic solvent extracts of flowers are very active against the tested bacterial stains when compared with leaf extracts.<span style="mso-spacerun: yes;">  </span>Among the entire extracts petroleum ether flower extract showed high activity (11-13 mm zone of inhibition) on all the tested organisms.<span style="mso-spacerun: yes;">  </span>Petroleum ether leaf and flower extracts gave very low MIC values. Petroleum ether flower extract gave lowest MIC values (0.390 to 1.562 µg/ml) against all the bacterial isolates tested.</span></p> ER -