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TY - JOUR AU - Kapoor, Rashmi AU - Nasim, Sekh Abdul AU - Mahmooduzzafar, M. AU - Mujib, Abdul PY - 2012/03/30 Y2 - 2025/09/23 TI - Establishment of efficient method for callus culture and shoot regeneration of local Indian garlic (var. Yamuna safed) JF - Journal of Ecobiotechnology JA - JEBT VL - 3 IS - 12 SE - REGULAR ISSUE ARTICLES DO - UR - https://www.updatepublishing.com/journal/index.php/jebt/article/view/157 SP - AB - <p class="MsoNormal" style="text-align: justify; text-indent: 36pt; margin: 0cm 0cm 0pt; text-autospace: ideograph-numeric;"><span style="font-size: small;"><span style="font-size: x-small;"><span style="font-family: arial,helvetica,sans-serif;"><span lang="EN-US">The experiment was designed to investigate the regeneration potentiality of a garlic variety (Yamuna Safed (G1)), and also to develop an efficient protocol for regeneration of garlic via callus culture. Higher percentage of callus was initiated from the combination of </span><span style="mso-bidi-font-family: Times-Roman;" lang="EN-US">BAP and 2, 4 D at 2.0 mg/l and 0.25 mg/l respectively</span><span lang="EN-US">.<span style="mso-spacerun: yes;">  </span>The hormone 2, 4-D, commonly used in tissue culture in garlic was found to be detrimental when used at the levels described in literatures. <span style="color: black;">Rooting o</span>f individual shoots was induced after transfer to medium without growth regulator. The plantlets were established in the soil after acclimatization. <span style="mso-spacerun: yes;"> </span></span></span></span></span></p> ER -