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TY - JOUR AU - S. Ohga, C.P. Pokhrel, R.K.P. Yadav, PY - 2009/12/26 Y2 - 2025/09/23 TI - Effects of physical factors and synthetic media on mycelial growth of Lyophyllum decastes JF - Journal of Ecobiotechnology JA - JEBT VL - 1 IS - 1 SE - REGULAR ISSUE ARTICLES DO - UR - https://www.updatepublishing.com/journal/index.php/jebt/article/view/10 SP - AB - <span style="font-size: 10pt; line-height: 110%; font-family: ";Garamond";,";serif";; mso-fareast-font-family: SimSun; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA; mso-bidi-font-weight: bold; mso-bidi-font-style: italic;">We investigated the effects of light, moisture, amino acids, vitamins and mineral nutrients on mycelial growth of the medicinal mushroom <em>Lyophyllum decastes</em>. Mycelial growth experiments were carried out on solid media. The best mycelial extension was recorded in total dark conditions, followed by 12 h alternating light/dark and total light, in respective order. This fungus grew well on substrate containing 65% moisture. Of the eight amino acids tested, the best growth (61.73 mm, after two-week incubation) was supported by glutamic acid, followed by proline and alanine. Six vitamin sources were used and riboflavin was the most utilizable (56.57mm). CaSO₄ was found to be the most favorable mineral source for growth (67.80 mm).</span> ER -