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@article{P. Ramesh Kannan, R. Rengasamy_2010, title={Optimization of Culture Conditions for the Production of Protease from Bacillus megaterium}, volume={2}, url={https://www.updatepublishing.com/journal/index.php/jebt/article/view/58}, abstractNote={<span style="font-size: 10pt; font-family: ";Garamond";,";serif";;"><p class="Abstract" style="margin: 0in -1.8pt 0pt 0in; tab-stops: center 2.75in right 5.5in left 472.5pt;"><strong><span style="font-size: 12pt; font-family: ";Garamond";,";serif";;"><em>Abstract</em></span></strong></p><p class="MsoNormal" style="margin: 0in 0in 0pt; text-indent: 0in; line-height: normal;"><span style="font-size: 10pt; font-family: ";Garamond";,";serif";; mso-bidi-font-weight: bold;"></span><span style="font-size: 10pt; font-family: ";Garamond";,";serif";;">In this Study, <em>Bacillus megaterium</em> was used for protease production to examine the optimal condition for its maximum accumulation. Hence the production of enzyme was investigated at various pH, temperature, NaCl concentrations and different carbon and nitrogen sources. The medium amended with 3.0% NaCl, 1.0% gelatin, 0. 4% lactose, 0.5% peptone and pH 9, incubation at 40°C for 42 h were supported maximum protease production of 78.5 U/ml. </span><span style="font-size: 12pt;"></span></p><p class="MsoNormal" style="margin: 0in 0in 0pt; text-indent: 0in; line-height: normal; text-align: left;" align="left"><em>---</em></p><p class="MsoNormal" style="margin: 0in 0in 0pt; text-indent: 0in; line-height: normal; text-align: left;" align="left"><em>Centre for Advanced studies in Botany, University of Madras, Guindy campus, Chennai- 600 025, India</em></p><p class="MsoNormal" style="margin: 0in 0in 0pt; text-indent: 0in; line-height: normal; text-align: left;" align="left"><em>---</em></p><p class="MsoNormal" style="margin: 0in 0in 0pt 0.25in; text-indent: -0.25in; line-height: normal;"><span style="text-decoration: underline;"><span style="font-family: ";Garamond";,";serif";; mso-bidi-font-size: 10.5pt;"><span style="font-size: small;">Please Cite This Article As: </span></span></span></p><p class="MsoNormal" style="margin: 0in 0in 0pt; text-indent: 0in; line-height: normal;"><span style="font-family: ";Garamond";,";serif";; mso-bidi-font-size: 10.5pt;"><span style="font-size: small;"> </span></span></p><p class="MsoNormal" style="margin: 0in 0in 0pt; text-indent: 0in; line-height: normal;"><span style="font-family: ";Garamond";,";serif";; mso-bidi-font-size: 10.5pt;"><span style="font-size: small;">R. Rajkumar*, K. R. Jayappriyan, P. Ramesh Kannan, R. Rengasamy. 2010. Optimization of Culture Conditions for the Production of Protease from <em>Bacillus megaterium</em>. J. Ecobiotechnol. 2(4):40-46.</span></span></p><p class="MsoNormal" style="margin: 0in 0in 0pt; text-indent: 0in; line-height: normal; text-align: left;" align="left"> </p></span>}, number={4}, journal={Journal of Ecobiotechnology}, author={P. Ramesh Kannan, R. Rengasamy, R. Rajkumar*, K. R. Jayappriyan,}, year={2010}, month={Oct.} }