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TY  - JOUR
AU  - Kwon, Do Yeon
AU  - Lim, Jinsu
AU  - Sathasivam, Ramaraj
AU  - Uddin, Md. Romij
AU  - Kim, Jae Kwang
AU  - Park, Sang Un
PY  - 2024/02/27
Y2  - 2025/09/23
TI  - Molecular characterization of diosgenin biosynthesis in different organs of Trigonella foenum-graecum L.
JF  - Journal of Aridland Agriculture
JA  - jaa
VL  - 10
IS  - 
SE  - Articles
DO  - 10.25081/jaa.2024.v10.8811
UR  - https://www.updatepublishing.com/journal/index.php/jaa/article/view/8811
SP  - 15-19
AB  - &lt;p&gt;Fenugreek, an annual herb, is a significant source of diosgenin, a triterpene with bioactive properties. This study aimed to molecularly characterize diosgenin biosynthesis in various organs of fenugreek, including flowers, immature leaves, developing leaves, mature leaves, stems, roots, and pods. The biosynthetic pathway involves vital enzymes such as squalene synthase, squalene monooxygenase, cycloartenol synthase, and Sterol-3-ß-glucosyl transferase. The study found that flowers and roots exhibited the highest gene expression levels, indicating their potential significance in diosgenin biosynthesis. Diosgenin content was quantified using high-performance liquid chromatography, with seeds being the primary source. The findings suggest that fenugreek offers a cost-effective and rapid alternative to yam for diosgenin production, and the differential accumulation of diosgenin in different plant organs underscores the importance of understanding organ-specific biosynthesis. This comprehensive analysis provides valuable insights into fenugreek’s potential as a bioresource for diosgenin and its diverse applications in medicine and agriculture.&lt;/p&gt;
ER  -