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@article{Ilyas_2011, title={Strain improvement of Phosphate solubilizing fungal strains for the production of lipase}, volume={1}, url={https://www.updatepublishing.com/journal/index.php/imrj/article/view/1495}, abstractNote={<span style="font-size: 10pt; font-family: ";Times New Roman";,";serif";; position: relative; top: 5pt; mso-fareast-font-family: BatangChe; mso-text-raise: -5.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-no-proof: yes;">A laboratory study was conducted to isolate, investigate and enhance the phosphate solubilization efficiency of certain isolated fungal strains viz., <em>Aspergillus niger, Aspergillus fumigatus, Penicillium sp</em>. These fungal strains were isolated from the rhizosphere of paddy fields in Tamilnadu, India and screened for phosphate solubilization. The </span><span style="font-size: 10pt; font-family: ";Times New Roman";,";serif";; position: relative; top: 5pt; mso-fareast-font-family: Calibri; mso-text-raise: -5.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-no-proof: yes; mso-bidi-font-weight: bold;">enhancement of Lipase activity </span><span style="font-size: 10pt; font-family: ";Times New Roman";,";serif";; position: relative; top: 5pt; mso-fareast-font-family: BatangChe; mso-text-raise: -5.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-no-proof: yes;">of these strains was studied through mutagenesis: UV, sodium azide and ethyl methane sulphonate. The mutant ANuv50 exhibited 32.72% increased efficiency for </span><span style="font-size: 10pt; font-family: ";Times New Roman";,";serif";; position: relative; top: 5pt; mso-fareast-font-family: Calibri; mso-text-raise: -5.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-no-proof: yes; mso-bidi-font-weight: bold;">Lipase activity in the presence of sucrose as a carbon source and 91.49%</span><span style="font-size: 10pt; font-family: ";Times New Roman";,";serif";; position: relative; top: 5pt; mso-fareast-font-family: BatangChe; mso-text-raise: -5.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-no-proof: yes;"><span style="mso-spacerun: yes;"> </span>increased efficiency for </span><span style="font-size: 10pt; font-family: ";Times New Roman";,";serif";; position: relative; top: 5pt; mso-fareast-font-family: Calibri; mso-text-raise: -5.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-no-proof: yes; mso-bidi-font-weight: bold;">Lipase activity in the presence of olive oil as a carbon source</span><span style="font-size: 10pt; font-family: ";Times New Roman";,";serif";; position: relative; top: 5pt; mso-fareast-font-family: BatangChe; mso-text-raise: -5.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-no-proof: yes;"><span style="mso-spacerun: yes;"> </span>compared to the wild strains. Similarly, the </span><span style="font-size: 10pt; font-family: ";Times New Roman";,";serif";; position: relative; top: 5pt; mso-fareast-font-family: Calibri; mso-text-raise: -5.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-no-proof: yes; mso-bidi-font-weight: bold;">Lipase activity </span><span style="font-size: 10pt; font-family: ";Times New Roman";,";serif";; position: relative; top: 5pt; mso-fareast-font-family: BatangChe; mso-text-raise: -5.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-no-proof: yes;">by sodium azide mutant (ANsa90) was 61.11% higher </span><span style="font-size: 10pt; font-family: ";Times New Roman";,";serif";; position: relative; top: 5pt; mso-fareast-font-family: Calibri; mso-text-raise: -5.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-no-proof: yes; mso-bidi-font-weight: bold;">in the presence of sucrose as a carbon source, 107.60%</span><span style="font-size: 10pt; font-family: ";Times New Roman";,";serif";; position: relative; top: 5pt; mso-fareast-font-family: BatangChe; mso-text-raise: -5.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-no-proof: yes;"><span style="mso-spacerun: yes;">  </span>higher </span><span style="font-size: 10pt; font-family: ";Times New Roman";,";serif";; position: relative; top: 5pt; mso-fareast-font-family: Calibri; mso-text-raise: -5.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-no-proof: yes; mso-bidi-font-weight: bold;">in the presence of olive oil as a carbon source</span><span style="font-size: 10pt; font-family: ";Times New Roman";,";serif";; position: relative; top: 5pt; mso-fareast-font-family: BatangChe; mso-text-raise: -5.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-no-proof: yes;"><span style="mso-spacerun: yes;"> </span>and EMS mutant<span style="mso-spacerun: yes;">  </span>(ANems150) was 137.65% higher<span style="mso-spacerun: yes;">  </span></span><span style="font-size: 10pt; font-family: ";Times New Roman";,";serif";; position: relative; top: 5pt; mso-fareast-font-family: Calibri; mso-text-raise: -5.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-no-proof: yes; mso-bidi-font-weight: bold;">in the presence of sucrose as a carbon source, 98.93%</span><span style="font-size: 10pt; font-family: ";Times New Roman";,";serif";; position: relative; top: 5pt; mso-fareast-font-family: BatangChe; mso-text-raise: -5.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-no-proof: yes;"><span style="mso-spacerun: yes;">  </span>higher </span><span style="font-size: 10pt; font-family: ";Times New Roman";,";serif";; position: relative; top: 5pt; mso-fareast-font-family: Calibri; mso-text-raise: -5.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-no-proof: yes; mso-bidi-font-weight: bold;">in the presence of olive oil as a carbon source</span><span style="font-size: 10pt; font-family: ";Times New Roman";,";serif";; position: relative; top: 5pt; mso-fareast-font-family: BatangChe; mso-text-raise: -5.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-no-proof: yes;"><span style="mso-spacerun: yes;"> </span>than the wild strain. The efficient strains were further studied for the effect of carbon and nitrogen source on </span><span style="font-size: 10pt; font-family: ";Times New Roman";,";serif";; position: relative; top: 5pt; mso-fareast-font-family: Calibri; mso-text-raise: -5.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-no-proof: yes; mso-bidi-font-weight: bold;">Lipase activity</span><span style="font-size: 10pt; font-family: ";Times New Roman";,";serif";; position: relative; top: 5pt; mso-fareast-font-family: BatangChe; mso-text-raise: -5.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-no-proof: yes;">. Significance of the findings is discussed in detail. </span>}, number={7}, journal={International Multidisciplinary Research Journal}, author={Ilyas, Jayaraman Rajeshkumar and *M.H. Muhammad}, year={2011}, month={Sep.} }