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TY - JOUR AU - Sarkar, Md. Taufiqur Rahman AU - Rahman, Md. Mizanur AU - Alam, Mohammad Firoz PY - 2022/02/25 Y2 - 2025/09/23 TI - Direct in vitro organogenesis from leaf and internode of Coccinia cordifolia (L.) Cogn. JF - Horticultural Biotechnology Research JA - HBR VL - 8 IS - SE - Research Articles DO - 10.25081/hbr.2022.v8.7470 UR - https://www.updatepublishing.com/journal/index.php/hbr/article/view/7470 SP - 1-8 AB - <p>The current study was carried out to elucidate a reproducible protocol to develop plants directly from leaf and internodes to facilitate the genetic transformation in <em>Coccinia cordifolia</em> (L.) Cogn., a medicinal plant of the cucurbitaceae family. <em>In vitro</em> grown leaf and internodes were used, which were collected for regenerated shoots from field-grown nodal segments that were sterilized by 0.1% HgCl₂ treatment for 6 minutes. The nodal segment cultured on BAP, Kn and BAP, NAA combination where 1.5 mg/L BAP solitary supplement and augmented supplement with 0.1 mg/L NAA was most effective as 80% shoots regenerated with 4.0±0.37 and 2.7±0.45 shoots per culture, respectively. Collected leaf and internode responded 90% at 1.5 mg/L BAP + 0.1 mg/L NAA fortified full-strength MS medium. The highest number of shoots also regenerated in the same combination which were 8.1 ± 0.30 and 10.2±0.40, respectively and found internode as the best explant for direct organogenesis. For root induction, half strength of MS medium supplemented with 0.1 mg/L IBA was found most effective. The highest number of roots regenerated per shoot (6.8±0.10) and root length (2.8±0.20). The successful acclimatization of the <em>in vitro </em>(80%) grown plantlets proved the validity of the developed protocol of using biotechnological techniques for improving the plant.</p> ER -